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KRT14\n                    <jats:sup>+<\/jats:sup>\n                    progenitor cells are found to be the main progenitors for maintaining the ductal homeostasis of the submandibular SGs. In this study, we investigated the role of ATG5 in SG KRT14\n                    <jats:sup>+<\/jats:sup>\n                    cells in mice and humans. Human labial salivary glands (LSG) from primary Sjogren's syndrome (pSS) and non\u2010pSS patients (normal), and submandibular glands (SMG) from\n                    <jats:italic>Atg5<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>flox\/flox<\/jats:italic>\n                    <\/jats:sup>\n                    <jats:italic>; Krt14\u2010Cre<\/jats:italic>\n                    (cKO) mice were used. ATG5\n                    <jats:sup>+<\/jats:sup>\n                    KRT14\n                    <jats:sup>+<\/jats:sup>\n                    and p62\n                    <jats:sup>+<\/jats:sup>\n                    KRT14\n                    <jats:sup>+<\/jats:sup>\n                    cells were detected by immunofluorescence staining in LSG. TUNEL, immunofluorescence, immunohistochemistry, and western blot were performed to detect cell death in SMG. Saliva was collected in 12\u2010week\u2010old (12\u2009W) and 32\u2010week\u2010old (32\u2009W) mice, then the concentration of calcium and buffering capacity were detected to analyze the function of SG. We found that LSG from pSS patients showed increased p62 and decreased ATG5 in KRT14\n                    <jats:sup>+<\/jats:sup>\n                    cells. We further revealed that in 32\u2009W, (1) the function of salivary glands was significantly impaired in cKO mice, (2) cell death increased in cKO mice, but cl\u2010Caspase 3 was not significantly changed, and (3) cleaved gasdermin D increased and was highly expressed in KRT14\n                    <jats:sup>+<\/jats:sup>\n                    cells of cKO mice. After applying a pyroptosis inhibitor to 32\u2009W mice, the reduced saliva flow rate was rescued. In addition, pyroptosis was also found in KRT14\n                    <jats:sup>+<\/jats:sup>\n                    cells of pSS patients. Collectively, our results indicate that\n                    <jats:italic>Atg5<\/jats:italic>\n                    deficiency would induce pyroptosis in mice SG, which could lead to functional impairments of SG.\n                  <\/jats:p>","DOI":"10.1096\/fj.202200946r","type":"journal-article","created":{"date-parts":[[2022,11,7]],"date-time":"2022-11-07T09:59:31Z","timestamp":1667815171000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Loss of\n                    <scp>ATG5<\/scp>\n                    in\n                    <scp>KRT14<\/scp>\n                    <sup>+<\/sup>\n                    cells leads to accumulated functional impairments of salivary glands via pyroptosis"],"prefix":"10.1096","volume":"36","author":[{"given":"Siqi","family":"Yu","sequence":"first","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education, School &amp; Hospital of Stomatology Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haisheng","family":"Wang","sequence":"additional","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education, School &amp; Hospital of Stomatology Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Liu","sequence":"additional","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education, School &amp; Hospital of Stomatology Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Pei","sequence":"additional","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education, School &amp; Hospital of Stomatology Wuhan University  Wuhan China"},{"name":"Department of Cariology &amp; Endodontics, School and Hospital of Stomatology Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huan","family":"Liu","sequence":"additional","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education, School &amp; Hospital of Stomatology Wuhan University  Wuhan China"},{"name":"Department of Periodontics, School and Hospital of Stomatology Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiali","family":"Zhang","sequence":"additional","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education, School &amp; Hospital of Stomatology Wuhan University  Wuhan China"},{"name":"Department of Oral Pathology, School and Hospital of Stomatology Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9049-8807","authenticated-orcid":false,"given":"Lu","family":"Zhang","sequence":"additional","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education, School &amp; Hospital of Stomatology Wuhan University  Wuhan China"},{"name":"Department of Cariology &amp; Endodontics, School and Hospital of Stomatology Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4825-7772","authenticated-orcid":false,"given":"Qiuhui","family":"Li","sequence":"additional","affiliation":[{"name":"The State Key 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