{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T16:22:02Z","timestamp":1761668522721,"version":"build-2065373602"},"reference-count":58,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,12,30]],"date-time":"2022-12-30T00:00:00Z","timestamp":1672358400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100020771","name":"Natural Science Foundation for Young Scientists of Shanxi Province","doi-asserted-by":"publisher","award":["31900474"],"award-info":[{"award-number":["31900474"]}],"id":[{"id":"10.13039\/501100020771","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Mitosis entails global and dramatic alterations, such as higher\u2010order chromatin organization disruption, concomitant with global transcription downregulation. Cells reliably re\u2010establishing gene expression patterns upon mitotic exit and maintaining cellular identities remain poorly understood. Previous studies indicated that certain transcription factors (TFs) remain associated with individual loci during mitosis and serve as mitotic bookmarkers. However, it is unclear which regulatory factors remain bound to the compacted mitotic chromosomes. We developed formaldehyde\u2010assisted isolation of regulatory elements\u2010coupled mass spectrometry (FAIRE\u2010MS) that combines FAIRE\u2010based open chromatin\u2010associated protein pull\u2010down and mass spectrometry (MS) to quantify the open chromatin\u2010associated proteome during the interphase and mitosis. We identified 189 interphase and mitosis maintained (IM) regulatory factors using FAIRE\u2010MS and found intrinsically disordered proteins and regions (IDP(R)s) are highly enriched, which plays a crucial role in liquid\u2013liquid phase separation (LLPS) and chromatin organization during the cell cycle. Notably, in these IDP(R)s, we identified mitotic bookmarkers, such as CEBPB, HMGB1, and TFAP2A, and several factors, including MAX, HMGB3, hnRNP A2\/B1, FUS, hnRNP D, and TIAL1, which are at least partially bound to the mitotic chromosome. Furthermore, it will be essential to study whether these IDP(R)s through LLPS helps cells transit from mitosis to the G1 phase during the cell cycle.<\/jats:p>","DOI":"10.1096\/fj.202201038rrr","type":"journal-article","created":{"date-parts":[[2022,12,30]],"date-time":"2022-12-30T10:29:31Z","timestamp":1672396171000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["<scp>FAIRE\u2010MS<\/scp>\n                    reveals mitotic retention of transcriptional regulators on a proteome\u2010wide scale"],"prefix":"10.1096","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6772-5063","authenticated-orcid":false,"given":"Bingyu","family":"Ye","sequence":"first","affiliation":[{"name":"Beijing Institute of Biotechnology  Beijing China"},{"name":"State Key Laboratory of Cell Differentiation and Regulation, College of Life Sciences Henan Normal University  Xinxiang 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