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As a complex pathological process, the etiology of RSA remains poorly understood. Recent studies have established that gene expression changes dramatically in human endometrial stromal cells (ESCs) during decidualization. N6\u2010methyladenosine (m\n                    <jats:sup>6<\/jats:sup>\n                    A) modification is the most prevalent epigenetic modification of mRNA in eukaryotic cells and it is closely related to the occurrence and development of many pathophysiological phenomena. In this study, we first confirmed that high levels of m\n                    <jats:sup>6<\/jats:sup>\n                    A mRNA methylation in decidual tissues are associated with RSA. Then, we used m\n                    <jats:sup>6<\/jats:sup>\n                    A\u2010modified RNA immunoprecipitation sequence (m\n                    <jats:sup>6<\/jats:sup>\n                    A\u2010seq) and RNA sequence (RNA\u2010seq) to identify the differentially expressed m\n                    <jats:sup>6<\/jats:sup>\n                    A methylation in decidual tissues from RSA patients and identified the key genes involved in abnormal decidualization by bioinformatics analysis. Using m\n                    <jats:sup>6<\/jats:sup>\n                    A\u2010seq, we identified a total of 2169 genes with differentially expressed m\n                    <jats:sup>6<\/jats:sup>\n                    A methylation, of which 735 m\n                    <jats:sup>6<\/jats:sup>\n                    A hypermethylated genes and 1434 m\n                    <jats:sup>6<\/jats:sup>\n                    A hypomethylated genes were identified. Further joint analysis of m\n                    <jats:sup>6<\/jats:sup>\n                    A\u2010seq and RNA\u2010seq revealed that 133 genes were m\n                    <jats:sup>6<\/jats:sup>\n                    A modified with mRNA expression. GO and KEGG analyses indicated that these unique genes were mainly enriched in environmental information processing pathways, including the cytokine\u2013cytokine receptor interaction and PI3K\u2010Akt signaling pathway. In summary, this study uncovered the transcriptome\u2010wide m\n                    <jats:sup>6<\/jats:sup>\n                    A modification pattern in decidual tissue of RSA, which provides a theoretical basis for further research into m\n                    <jats:sup>6<\/jats:sup>\n                    A modification and new therapeutic strategies for RSA.\n                  <\/jats:p>","DOI":"10.1096\/fj.202201232rrrr","type":"journal-article","created":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T09:58:16Z","timestamp":1676368696000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Transcriptome\u2010wide high\u2010throughput m\n                    <sup>6<\/sup>\n                    A sequencing of differential\n                    <scp>\n                      m\n                      <sup>6<\/sup>\n                      A\n                    <\/scp>\n                    methylation patterns in the decidual tissues from\n                    <scp>RSA<\/scp>\n                    patients"],"prefix":"10.1096","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4689-536X","authenticated-orcid":false,"given":"Yong","family":"Luo","sequence":"first","affiliation":[{"name":"Department of Pharmacology, School of Basic Medical Sciences, Health Science Center Xi'an Jiaotong University  Xi'an China"},{"name":"Central Laboratory, Jiangxi Provincial Maternal and Child Health Hospital  Nanchang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Traditional Chinese Medicine, Jiangxi Provincial Maternal and Child Health Hospital  Nanchang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Traditional Chinese Medicine, Jiangxi Provincial Maternal and Child Health Hospital  Nanchang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Fang","sequence":"additional","affiliation":[{"name":"Department of Traditional Chinese Medicine, Jiangxi Provincial Maternal and Child Health Hospital  Nanchang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziyu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pathology, Jiangxi Provincial Maternal and Child Health Hospital  Nanchang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lili","family":"Hu","sequence":"additional","affiliation":[{"name":"Ambulatory Surgery Center, Jiangxi Provincial Maternal and Child Health Hospital  Nanchang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyong","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Traditional Chinese Medicine, Jiangxi Provincial Maternal and Child Health Hospital  Nanchang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zengming","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, School of Basic Medical Sciences, Health Science Center Xi'an Jiaotong University  Xi'an China"},{"name":"Central Laboratory, Jiangxi Provincial Maternal and Child Health Hospital  Nanchang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liping","family":"Li","sequence":"additional","affiliation":[{"name":"Prenatal Diagnosis Center, Key Laboratory of Women's Reproductive Health of Jiangxi Province, Jiangxi Provincial Maternal and Child Health Hospital  Nanchang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lina","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, School of Basic Medical Sciences, Health Science Center Xi'an Jiaotong University  Xi'an China"},{"name":"Key Laboratory of Environment and Genes Related to Diseases, 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