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Nevertheless, there are few reports on its action on intestinal ischemia\/reperfusion (I\/R). In this study, we mainly explored the effect of Bryo\u20101 on intestinal I\/R injury and determined the mechanism. C57BL\/6J mice underwent temporary superior mesenteric artery (SMA) obturation to induce I\/R, on the contrary, Caco\u20102 cells suffered to oxygen and glucose deprivation\/reperfusion (OGD\/R) to establish the in vitro model. RAW264.7 cells were stimulated with LPS to induce macrophage inflammation. The drug gradient experiment was used to demonstrate in vivo and in vitro models. Bryo\u20101 ameliorated the intestinal I\/R\u2010induced injury of multiple organs and epithelial cells. It also alleviated intestinal I\/R\u2010induced barrier disruption of intestines according to the histology, intestinal permeability, intestinal bacterial translocation rates, and tight junction protein expression results. Bryo\u20101 significantly inhibited oxidative stress damages and inflammation, which may contribute to the restoration of intestinal barrier function. Further, Bryo\u20101 significantly activated Nrf2\/HO\u20101 signaling in vivo. However, the deletion of Nrf2 in Caco\u20102 and RAW264.7 cells attenuated the protective functions of Bryo\u20101 and significantly abolished the anti\u2010inflammatory effect of Bryo\u20101 on LPS\u2010induced macrophage inflammation. Bryo\u20101 protects intestines against I\/R\u2010induced injury. It is associated with intestinal barrier protection, as well as inhibition of inflammation and oxidative stress partly through Nrf2\/HO\u20101 signaling.<\/jats:p>","DOI":"10.1096\/fj.202201540r","type":"journal-article","created":{"date-parts":[[2023,5,2]],"date-time":"2023-05-02T12:26:10Z","timestamp":1683030370000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Bryostatin\u20101 attenuates intestinal ischemia\/reperfusion\u2010induced intestinal barrier dysfunction, inflammation, and oxidative stress via activation of Nrf2\/HO\u20101 signaling"],"prefix":"10.1096","volume":"37","author":[{"given":"Mulin","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Gastrointestinal Surgery First Affiliated Hospital of Bengbu Medical College  Bengbu Anhui 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