{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T01:57:08Z","timestamp":1793239028101,"version":"4.5.0"},"reference-count":33,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,3,24]],"date-time":"2023-03-24T00:00:00Z","timestamp":1679616000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006313","name":"Barncancerfonden","doi-asserted-by":"publisher","award":["PR2018\u20100148"],"award-info":[{"award-number":["PR2018\u20100148"]}],"id":[{"id":"10.13039\/501100006313","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006313","name":"Barncancerfonden","doi-asserted-by":"publisher","award":["PR2021\u20100122"],"award-info":[{"award-number":["PR2021\u20100122"]}],"id":[{"id":"10.13039\/501100006313","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002794","name":"Cancerfonden","doi-asserted-by":"publisher","award":["CAN 2017\/502"],"award-info":[{"award-number":["CAN 2017\/502"]}],"id":[{"id":"10.13039\/501100002794","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002794","name":"Cancerfonden","doi-asserted-by":"publisher","award":["20 1008 PjF"],"award-info":[{"award-number":["20 1008 PjF"]}],"id":[{"id":"10.13039\/501100002794","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002794","name":"Cancerfonden","doi-asserted-by":"publisher","award":["20 1010 UsF"],"award-info":[{"award-number":["20 1010 UsF"]}],"id":[{"id":"10.13039\/501100002794","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000942","name":"Brain Foundation","doi-asserted-by":"publisher","award":["FO2022\u20100366"],"award-info":[{"award-number":["FO2022\u20100366"]}],"id":[{"id":"10.13039\/501100000942","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004063","name":"Knut och Alice Wallenbergs Stiftelse","doi-asserted-by":"publisher","award":["KAW 2019.0088"],"award-info":[{"award-number":["KAW 2019.0088"]}],"id":[{"id":"10.13039\/501100004063","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004359","name":"Vetenskapsr\u00e5det","doi-asserted-by":"publisher","award":["Dnr 2020\u201002563"],"award-info":[{"award-number":["Dnr 2020\u201002563"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Regulation of vascular permeability to plasma is essential for tissue and organ homeostasis and is mediated by endothelial cell\u2010to\u2010cell junctions that tightly regulate the trafficking of molecules between blood and tissue. The single\u2010pass transmembrane glycoprotein CD93 is upregulated in endothelial cells during angiogenesis and controls cytoskeletal dynamics. However, its role in maintaining homeostasis by regulating endothelial barrier function has not been elucidated yet. Here, we demonstrate that CD93 interacts with vascular endothelial (VE)\u2010cadherin and limits its phosphorylation and turnover. CD93 deficiency in vitro and in vivo induces phosphorylation of VE\u2010cadherin under basal conditions, displacing it from endothelial cell\u2013cell contacts. Consistent with this, endothelial junctions are defective in CD93\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice, and the blood\u2013brain barrier permeability is enhanced. Mechanistically, CD93 regulates VE\u2010cadherin phosphorylation and turnover at endothelial junctions through the Rho\/Rho kinase\u2010dependent pathway. In conclusion, our results identify CD93 as a key regulator of VE\u2010cadherin stability at endothelial junctions, opening up possibilities for therapeutic strategies directed to control vascular permeability.\n                  <\/jats:p>","DOI":"10.1096\/fj.202201623rr","type":"journal-article","created":{"date-parts":[[2023,3,24]],"date-time":"2023-03-24T10:45:34Z","timestamp":1679654734000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["<scp>CD93<\/scp>\n                    maintains endothelial barrier function by limiting the phosphorylation and turnover of\n                    <scp>VE<\/scp>\n                    \u2010cadherin"],"prefix":"10.1096","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7294-345X","authenticated-orcid":false,"given":"Roberta","family":"Lugano","sequence":"first","affiliation":[{"name":"Department of Immunology, Genetics and Pathology, Science for Life Laboratory Uppsala University  The Rudbeck Laboratory 75185 Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kalyani","family":"Vemuri","sequence":"additional","affiliation":[{"name":"Department of Immunology, Genetics and Pathology, Science for Life Laboratory Uppsala University  The Rudbeck Laboratory 75185 Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefano","family":"Barbera","sequence":"additional","affiliation":[{"name":"Department of Immunology, Genetics and Pathology, Science for Life Laboratory Uppsala University  The Rudbeck Laboratory 75185 Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maurizio","family":"Orlandini","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Chemistry and Pharmacy University of Siena  Via A. Moro, 2 53100 Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabetta","family":"Dejana","sequence":"additional","affiliation":[{"name":"Department of Immunology, Genetics and Pathology, Science for Life Laboratory Uppsala University  The Rudbeck Laboratory 75185 Uppsala Sweden"},{"name":"Vascular Biology Unit FIRC Institute of Molecular Oncology  Milan 20129 Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lena","family":"Claesson\u2010Welsh","sequence":"additional","affiliation":[{"name":"Department of Immunology, Genetics and Pathology, Science for Life Laboratory Uppsala University  The Rudbeck Laboratory 75185 Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Dimberg","sequence":"additional","affiliation":[{"name":"Department of Immunology, Genetics and Pathology, Science for Life Laboratory Uppsala University  The Rudbeck Laboratory 75185 Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,3,24]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2020.11.006"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/21688370.2016.1154641"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-014-1843-7"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-014-1856-2"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvq086"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-31408-3"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.4072"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2013.06.004"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2005.02120.x"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-14-3636"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI97459"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2021.05.006"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms222212417"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.6.3406"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms2199"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/emmm.201302520"},{"issue":"16","key":"e_1_2_10_18_1","first-page":"3041","article-title":"A local VE\u2010cadherin and Trio\u2010based signaling complex stabilizes endothelial junctions through Rac1","volume":"128","author":"Timmerman I","year":"2015","journal-title":"J Cell Sci"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsif.2010.0419"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2824"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1486"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200306001"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20190062"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-pathol-012615-044506"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.14985"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1100803"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/imm.12974"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80068-0"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature25739"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2018.06.033"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-62693-6"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1922713117"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.RA119.001716"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201807210"}],"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.202201623RR","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1096\/fj.202201623RR","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.202201623RR","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T19:20:03Z","timestamp":1764357603000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fj.202201623RR"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,24]]},"references-count":33,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2023,4]]}},"alternative-id":["10.1096\/fj.202201623RR"],"URL":"https:\/\/doi.org\/10.1096\/fj.202201623rr","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"value":"0892-6638","type":"print"},{"value":"1530-6860","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,24]]},"assertion":[{"value":"2022-10-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-03-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-03-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e22894"}}