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Cells in TMJ cartilage are zonally arranged, earlier differentiated in the super zone and late differentiated in the deep zone. The purpose was to detect the zonal interdependence in TMJ cartilage under dental biomechanical stimulations. Here, we obtained the\n                    <jats:italic>Sox9<\/jats:italic>\n                    <jats:sup>CreER<\/jats:sup>\n                    ;\n                    <jats:italic>Rosa26<\/jats:italic>\n                    <jats:sup>tdTomato<\/jats:sup>\n                    and\n                    <jats:italic>Col10<\/jats:italic>\n                    <jats:sup>CreER<\/jats:sup>\n                    ;\n                    <jats:italic>Rosa26<\/jats:italic>\n                    <jats:sup>tdTomato<\/jats:sup>\n                    mice to label super zone Sox9\u2010expressing (Sox9\n                    <jats:sup>+<\/jats:sup>\n                    ) or deep zone Col10\u2010expressing (Col10\n                    <jats:sup>+<\/jats:sup>\n                    ) cells by tdTomato (TdT), and\n                    <jats:italic>Sox9<\/jats:italic>\n                    <jats:sup>CreER<\/jats:sup>\n                    ;\n                    <jats:italic>Rosa26<\/jats:italic>\n                    <jats:sup>DTA<\/jats:sup>\n                    and\n                    <jats:italic>Col10<\/jats:italic>\n                    <jats:sup>CreER<\/jats:sup>\n                    ;\n                    <jats:italic>Rosa26<\/jats:italic>\n                    <jats:sup>DTA<\/jats:sup>\n                    mice to ablate Sox9\n                    <jats:sup>+<\/jats:sup>\n                    or Col10\n                    <jats:sup>+<\/jats:sup>\n                    cells selectively. These mice were subjected to unilateral anterior crossbite (UAC) or bilateral anterior elevation (BAE) dental stimulation, which promoted terminal differentiation or proliferation of TMJ chondrocytes, respectively. In both UAC and BAE models, the Sox9\u2010TdT\n                    <jats:sup>+<\/jats:sup>\n                    cells performed as proliferation and mature differentiation, showing as expressing Ki67 and Col\u2010X, respectively; while the Col10\u2010TdT\n                    <jats:sup>+<\/jats:sup>\n                    cells performed as terminal differentiation, showing as expressing osteocalcin (OCN). In both Sox9\n                    <jats:sup>+<\/jats:sup>\n                    \u2010 and Col10\n                    <jats:sup>+<\/jats:sup>\n                    \u2010cells ablation groups, there were reductions in cell number, cartilage thickness and matrix amount, subchondral bone loss, and condylar deformation. The UAC\u2010promoted terminal differentiation was enhanced, and the BAE\u2010promoted cellular proliferation was ruined. Impressively, when Col10\n                    <jats:sup>+<\/jats:sup>\n                    cells were ablated, the UAC\u2010promoted DAP3 expression, an anoikis marker, was further increased, while the BAE\u2010suppressed DAP3 expression was instead greatly increased. These findings demonstrated that the cartilage zones function interdependently. The super zone harbors the cells that undergo differentiation to deep zone cells, the deep zone contains load\u2010bearing matrix which is structural essential for the cells located inside or superficial.\n                  <\/jats:p>","DOI":"10.1096\/fj.202201662rr","type":"journal-article","created":{"date-parts":[[2023,3,24]],"date-time":"2023-03-24T10:55:53Z","timestamp":1679655353000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Zonal interdependence in the temporomandibular joint cartilage"],"prefix":"10.1096","volume":"37","author":[{"given":"Xiaojie","family":"Xu","sequence":"first","affiliation":[{"name":"School of Stomatology The Third Affiliated Hospital of Xinxiang Medical University  Xinxiang China"},{"name":"College of Life Sciences Northwest University  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuejiao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases Shanghai Stomatological Hospital, Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Oral Anatomy and Physiology, School of Stomatology The Fourth Military Medical University  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongmei","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Stomatology The Third Affiliated Hospital of Xinxiang Medical University  Xinxiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongxu","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Oral Anatomy and Physiology, School of Stomatology The Fourth Military 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