{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T03:06:39Z","timestamp":1792292799202,"version":"4.4.0"},"reference-count":45,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,3,15]],"date-time":"2023-03-15T00:00:00Z","timestamp":1678838400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31800903"],"award-info":[{"award-number":["31800903"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Multiple sclerosis (MS) is an autoimmune disease characterized by inflammatory infiltration and demyelination in the central nervous system (CNS). IFN\u2010gamma (IFN\u2010\u03b3), a critically important immunomodulator, has been widely studied in MS pathology. The confusing and complex effects of IFN\u2010\u03b3 in MS patients and rodent models, however, cause us to look more closely at its exact role in MS. In this study, we identified the role of the IFN\u2010\u03b3 signaling in the choroid plexus (CP) in the experimental autoimmune encephalomyelitis (EAE) model. We found that the IFN\u2010\u03b3 signal was rapidly amplified when CNS immune cell infiltration occurred in the CP during the progressive stage. Furthermore, using two CP\u2010specific knockdown strategies, we demonstrated that blocking the IFN\u2010\u03b3 signal via knockdown of IFN\u2010\u03b3R1 in the CP could protect mice against EAE pathology, as evidenced by improvements in clinical scores and infiltration. Notably, knocking down IFN\u2010\u03b3R1 in the CP reduced the local expression of adhesion molecules and chemokines. This finding suggests that IFN\u2010\u03b3 signaling in the CP may participate in the pathological process of EAE by preventing pathological T helper (Th) 17\n                    <jats:sup>+<\/jats:sup>\n                    cells from infiltrating into the CNS. Finally, we showed that the unbalanced state of IFN\u2010\u03b3 signaling between peripheral lymphocytes and the choroid plexus may determine whether IFN\u2010\u03b3 has a protective or aggravating effect on EAE pathology. Above all, we discovered that IFN\u2010\u03b3R1\u2010mediated IFN\u2010\u03b3 signaling in the CP was a vital pathway in the pathological process of EAE.\n                  <\/jats:p>","DOI":"10.1096\/fj.202201767r","type":"journal-article","created":{"date-parts":[[2023,3,15]],"date-time":"2023-03-15T14:09:16Z","timestamp":1678889356000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Blocking the\n                    <scp>IFN<\/scp>\n                    \u2010gamma signal in the choroid plexus confers resistance to experimental autoimmune encephalomyelitis"],"prefix":"10.1096","volume":"37","author":[{"given":"Yuyin","family":"Zheng","sequence":"first","affiliation":[{"name":"Rehabilitation Medicine Center The Second Affiliated Hospital of Wenzhou Medical University  Wenzhou China"},{"name":"Integrative &amp; Optimized Medicine Research center, China\u2010USA Institute for Acupuncture and Rehabilitation Wenzhou Medical University  Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lanxin","family":"Hu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ophthalmology, Optometry and Visual Science, Eye Hospital Wenzhou Medical University  Wenzhou China"},{"name":"The Molecular Neuropharmacology Laboratory and the Eye\u2010Brain Research Center, School of Ophthalmology &amp; Optometry and Eye Hospital Wenzhou Medical University  Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuwen","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ophthalmology, Optometry and Visual Science, Eye Hospital Wenzhou Medical University  Wenzhou China"},{"name":"The Molecular Neuropharmacology Laboratory and the Eye\u2010Brain Research Center, School of Ophthalmology &amp; Optometry and Eye Hospital Wenzhou Medical University  Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng","family":"Zheng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ophthalmology, Optometry and Visual Science, Eye Hospital Wenzhou Medical University  Wenzhou China"},{"name":"The Molecular Neuropharmacology Laboratory and the Eye\u2010Brain Research Center, School of Ophthalmology &amp; Optometry and Eye Hospital Wenzhou Medical University  Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenzhan","family":"Tu","sequence":"additional","affiliation":[{"name":"Rehabilitation Medicine Center The Second Affiliated Hospital of Wenzhou Medical University  Wenzhou China"},{"name":"Integrative &amp; Optimized Medicine Research center, China\u2010USA Institute for Acupuncture and Rehabilitation Wenzhou Medical University  Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiyan","family":"Lin","sequence":"additional","affiliation":[{"name":"Rehabilitation Medicine Center The Second Affiliated Hospital of Wenzhou Medical University  Wenzhou China"},{"name":"Integrative &amp; Optimized Medicine Research center, China\u2010USA Institute for Acupuncture and Rehabilitation Wenzhou Medical University  Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haotian","family":"Wang","sequence":"additional","affiliation":[{"name":"Alberta Institute Wenzhou Medical University  Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiwei","family":"Jiang","sequence":"additional","affiliation":[{"name":"Alberta Institute Wenzhou Medical University  Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Songhe","family":"Jiang","sequence":"additional","affiliation":[{"name":"Rehabilitation Medicine Center The Second Affiliated Hospital of Wenzhou Medical University  Wenzhou China"},{"name":"Integrative &amp; Optimized Medicine Research center, China\u2010USA Institute for Acupuncture and Rehabilitation Wenzhou Medical University  Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5091-9694","authenticated-orcid":false,"given":"Wu","family":"Zheng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ophthalmology, Optometry and Visual Science, Eye Hospital Wenzhou Medical University  Wenzhou China"},{"name":"The Molecular Neuropharmacology Laboratory and the Eye\u2010Brain Research Center, School of Ophthalmology &amp; Optometry and Eye Hospital Wenzhou Medical University  Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,3,15]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/247551a0"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(15)00018-6"},{"issue":"12","key":"e_1_2_12_4_1","first-page":"S10","article-title":"Interferon beta\u2010lb is effective in relapsing\u2010remitting multiple sclerosis. II. MRI analysis results of a multicenter, randomized, double\u2010blind, placebo\u2010controlled trial. 1993 [classical article]","volume":"57","author":"Paty DW","year":"2001","journal-title":"Neurology"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2017.08.013"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.21652"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.3310\/hta21520"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2016.02.001"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.37.7.1097"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(87)92863-7"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1177\/135245850100700502"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/3-540-37673-9_1"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1006\/mcne.1998.0725"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.156.1.5"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.157.8.3223"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830260735"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.140.5.1506"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-5728(92)90042-J"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00004.2013"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.4161\/cam.21054"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00041.2017"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2013.02.012"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0029(20010101)52:1<112::AID-JEMT13>3.0.CO;2-5"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneuroim.2008.04.035"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004410051287"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40478-020-0885-1"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1001\/2013.jamaneurol.409"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaneurol.2014.395"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-022-02415-z"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1716"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2012.07807.x"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.04-2030fje"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.15252\/embj.201591468"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aav4111"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awt259"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1089\/hum.2019.300"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-020-02021-x"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2007.078527"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2010.01.014"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201444612"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.163.10.5278"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.1186\/1742-2094-9-7"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1303321"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-018-1276-4"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.1186\/1742-2094-9-193"},{"key":"e_1_2_12_46_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1505955112"}],"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.202201767R","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1096\/fj.202201767R","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.202201767R","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T19:21:16Z","timestamp":1764357676000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fj.202201767R"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,15]]},"references-count":45,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2023,4]]}},"alternative-id":["10.1096\/fj.202201767R"],"URL":"https:\/\/doi.org\/10.1096\/fj.202201767r","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"value":"0892-6638","type":"print"},{"value":"1530-6860","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,15]]},"assertion":[{"value":"2022-10-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-02-09","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-03-15","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e22833"}}