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Hyperglycemia, hyperlipidemia, and metabolic disorders are always accompanied by the majority of overweight patients. The association between granulosa cell function and metabolic alterations in follicular fluid including lipids, proteins, and growth factors has been extensively documented. However, the effects of higher glucose level on ovarian granulosa cells (GCs), remain largely unknown. In this study, we identified that overweight women had elevated follicular glucose level which profoundly activated NLRP3 inflammasome and pyroptosis. An in vitro correlation between follicular high glucose, NLRP3 inflammasome and pyroptosis was also established. More importantly, in granulosa cells of overweight patients, the activation of the NLRP3 inflammasome and pyroptosis induced by high glucose was involved in the dysregulation of estradiol synthesis. Our study may provide new options to interpretate and improve IVF outcomes in overweight women.<\/jats:p>","DOI":"10.1096\/fj.202201796rr","type":"journal-article","created":{"date-parts":[[2023,2,24]],"date-time":"2023-02-24T10:37:27Z","timestamp":1677235047000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Local glucose elevation activates pyroptosis via\n                    <scp>NLRP3<\/scp>\n                    inflammasome in ovarian granulosa cells of overweight patients"],"prefix":"10.1096","volume":"37","author":[{"given":"Rui","family":"Xu","sequence":"first","affiliation":[{"name":"Center for Reproductive Medicine, Ren Ji Hospital Shanghai Jiao Tong University School of Medicine  Shanghai China"},{"name":"Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics  Shanghai 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