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We aimed to test the\n                    <jats:italic>Arl15<\/jats:italic>\n                    gene as a functional candidate for metabolic traits in the mouse. CRISPR\/Cas9 germline knockout (KO) of\n                    <jats:italic>Arl15<\/jats:italic>\n                    showed that homozygotes were postnatal lethal and exhibited a complete cleft palate (CP). Also, decreased cell migration was observed from\n                    <jats:italic>Arl15<\/jats:italic>\n                    KO mouse embryonic fibroblasts (MEFs). Metabolic phenotyping of heterozygotes showed that females had reduced fat mass on a chow diet from 14\u2009weeks of age. Mild body composition phenotypes were also observed in heterozygous mice on a high\u2010fat diet (HFD)\/low\u2010fat diet (LFD). Females on a HFD showed reduced body weight, gonadal fat depot weight and brown adipose tissue (BAT) weight. In contrast, in the LFD group, females showed increased bone mineral density (BMD), while males showed a trend toward reduced BMD. Clinical biochemistry analysis of plasma on HFD showed transient lower adiponectin at 20\u2009weeks of age in females. Urinary and plasma Mg\n                    <jats:sup>2+<\/jats:sup>\n                    concentrations were not significantly different. Our phenotyping data showed that\n                    <jats:italic>Arl15<\/jats:italic>\n                    is essential for craniofacial development. Adult metabolic phenotyping revealed potential roles in brown adipose tissue and bone development.\n                  <\/jats:p>","DOI":"10.1096\/fj.202201918r","type":"journal-article","created":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T12:53:27Z","timestamp":1695992007000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Cleft palate and minor metabolic disturbances in a mouse global\n                    <i>Arl15<\/i>\n                    gene knockout"],"prefix":"10.1096","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1823-3382","authenticated-orcid":false,"given":"Ying","family":"Bai","sequence":"first","affiliation":[{"name":"Mammalian Genetics Unit and Mary Lyon Centre MRC Harwell Institute  Didcot Oxon UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liz","family":"Bentley","sequence":"additional","affiliation":[{"name":"Mammalian Genetics Unit and Mary Lyon Centre MRC Harwell Institute  Didcot Oxon UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chao","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Physiology, Radboud Institute for Molecular Life Sciences Radboud University Medical Centre  Nijmegen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Navaratnam","family":"Naveenan","sequence":"additional","affiliation":[{"name":"MRC London Institute of Medical Sciences  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James","family":"Cleak","sequence":"additional","affiliation":[{"name":"Mammalian Genetics Unit and Mary Lyon Centre MRC Harwell Institute  Didcot Oxon UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yixing","family":"Wu","sequence":"additional","affiliation":[{"name":"Mammalian Genetics Unit and Mary Lyon Centre MRC Harwell Institute  Didcot Oxon UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michelle M.","family":"Simon","sequence":"additional","affiliation":[{"name":"Mammalian Genetics Unit and Mary Lyon Centre MRC Harwell Institute  Didcot Oxon UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henrik","family":"Westerberg","sequence":"additional","affiliation":[{"name":"Mammalian Genetics Unit and Mary Lyon Centre MRC Harwell Institute  Didcot Oxon UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ram\u00f3n Casero","family":"Ca\u00f1as","sequence":"additional","affiliation":[{"name":"Mammalian Genetics Unit and Mary Lyon Centre MRC Harwell Institute  Didcot Oxon UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neil","family":"Horner","sequence":"additional","affiliation":[{"name":"Mammalian Genetics Unit and Mary Lyon Centre MRC Harwell Institute  Didcot Oxon UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rajesh","family":"Pandey","sequence":"additional","affiliation":[{"name":"Mammalian Genetics Unit and Mary Lyon Centre MRC Harwell Institute  Didcot Oxon UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keanu","family":"Paphiti","sequence":"additional","affiliation":[{"name":"Mammalian Genetics Unit and Mary Lyon Centre MRC Harwell Institute  Didcot Oxon UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulrike","family":"Schulze","sequence":"additional","affiliation":[{"name":"MRC Laboratory of Molecular Biology Cambridge Biomedical Campus  Cambridge UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joffrey","family":"Miann\u00e9","sequence":"additional","affiliation":[{"name":"Mammalian Genetics Unit and Mary Lyon Centre MRC Harwell Institute  Didcot Oxon UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tertius","family":"Hough","sequence":"additional","affiliation":[{"name":"Mammalian Genetics Unit and Mary Lyon Centre MRC Harwell Institute  Didcot Oxon UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lydia","family":"Teboul","sequence":"additional","affiliation":[{"name":"Mammalian Genetics Unit and Mary Lyon Centre MRC Harwell Institute  Didcot Oxon UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2372-8486","authenticated-orcid":false,"given":"Jeroen H. 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