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The transcriptome responses of different types of muscle fibers and mononuclear cells in skeletal muscle to muscular dystrophy caused by\n                    <jats:italic>Ptrf<\/jats:italic>\n                    deletion have not been explored. Here, we created muscular dystrophy mice by\n                    <jats:italic>Ptrf<\/jats:italic>\n                    knockout and applied single\u2010nucleus RNA sequencing (snRNA\u2010seq) to unveil the transcriptional changes of the skeletal muscle at single\u2010nucleus resolution. 11\u2009613 muscle nuclei (WT, 5838;\n                    <jats:italic>Ptrf<\/jats:italic>\n                    KO, 5775) were classified into 12 clusters corresponding to 11 nuclear types. Trajectory analysis revealed the potential transition between type IIb_1 and IIb_2 myonuclei upon muscular dystrophy. Functional enrichment analysis indicated that apoptotic signaling and enzyme\u2010linked receptor protein signaling pathway were significantly enriched in type IIb_1 and IIb_2 myonuclei of\n                    <jats:italic>Ptrf<\/jats:italic>\n                    KO, respectively. The muscle structure development and the PI3K\u2010AKT signaling pathway were significantly enriched in type IIa and IIx myonuclei of\n                    <jats:italic>Ptrf<\/jats:italic>\n                    KO. Meanwhile, metabolic pathway analysis showed a decrease in overall metabolic pathway activity of myonuclei subtypes upon muscular dystrophy, with the most decrease in type IIb_1 myonuclei. Gene regulatory network analysis found that the activity of Mef2c, Mef2d, Myf5, and Pax3 regulons was enhanced in type II myonuclei of\n                    <jats:italic>Ptrf<\/jats:italic>\n                    KO, especially in type IIb_2 myonuclei. In addition, we investigated the transcriptome changes in adipocytes and found that muscular dystrophy enhanced the lipid metabolic capacity of adipocytes. Our findings provide a valuable resource for exploring the molecular mechanism of muscular dystrophy due to\n                    <jats:italic>Ptrf<\/jats:italic>\n                    deficiency.\n                  <\/jats:p>","DOI":"10.1096\/fj.202201949rr","type":"journal-article","created":{"date-parts":[[2023,5,26]],"date-time":"2023-05-26T12:45:09Z","timestamp":1685105109000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Decoding the transcriptome of muscular dystrophy due to\n                    <i>Ptrf<\/i>\n                    deficiency using single\u2010nucleus\n                    <scp>RNA<\/scp>\n                    sequencing"],"prefix":"10.1096","volume":"37","author":[{"given":"Xiaokai","family":"Li","sequence":"first","affiliation":[{"name":"Livestock and Poultry Multi\u2010omics Key Laboratory of Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology Sichuan 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