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Pre\u2010messenger RNA (pre\u2010mRNA) processing is the basic mechanism required for gene expression, and members of the serine\/arginine\u2010rich protein (SR) family are key components of the machines that perform these basic processing events. Serine\/arginine\u2010rich splicing factor 2 (SRSF2) is an important member of the SR family; however, the physiological functions of SRSF2 in Sertoli cells are still unclear. Here, we found that SRSF2 was localized in the nuclei of Sertoli and germ cells in male mice at all stages by breeding\n                    <jats:italic>Amh\u2010Cre<\/jats:italic>\n                    mice obtained with\n                    <jats:italic>Srsf2<\/jats:italic>\n                    \u2010specific knockout in Sertoli cells to define the function of SRSF2 in Sertoli cells. The experimental results showed that specific deletion of SRSF2 impaired fetal Sertoli cell proliferation and induced abnormal apoptosis and severe DNA damage in seminiferous tubules, resulting in severe testicular dysplasia, seminiferous tubule atrophy, and almost no normal seminiferous tubules at postnatal day 14. Eventually, these changes resulted in failure to produce normal sperm and infertility. Further RNA\u2010seq results showed that many key genes related to proliferation and apoptosis were downregulated;\n                    <jats:italic>Racgap1<\/jats:italic>\n                    mRNA undergoes exon skipping. Thus, SRSF2\u2010dependent Sertoli cells are essential for testicular development and male reproduction.\n                  <\/jats:p>","DOI":"10.1096\/fj.202202152rr","type":"journal-article","created":{"date-parts":[[2023,4,11]],"date-time":"2023-04-11T10:57:25Z","timestamp":1681210645000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["SRSF2 in Sertoli cells is essential for testicular development and spermatogenesis in mice"],"prefix":"10.1096","volume":"37","author":[{"given":"Xiaomei","family":"Xie","sequence":"first","affiliation":[{"name":"State Key Laboratory of Agrobiotechnology College of Biological Sciences, China Agricultural University  Beijing 100193 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Longjie","family":"Sun","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agrobiotechnology College of Biological Sciences, China Agricultural University  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