{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,6]],"date-time":"2026-11-06T22:09:52Z","timestamp":1794002992511,"version":"4.8.0"},"reference-count":29,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2023,7,13]],"date-time":"2023-07-13T00:00:00Z","timestamp":1689206400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81920108013"],"award-info":[{"award-number":["81920108013"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82001071"],"award-info":[{"award-number":["82001071"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The superficial zone cells in mandibular condylar cartilage are proliferative. The present purpose was to delineate the relation of calcium\u2010sensing receptor (CaSR) and parathyroid hormone\u2010related peptide nuclear localization sequence (PTHrP\n                    <jats:sub>87\u2010139<\/jats:sub>\n                    ), and their role in the proliferation behaviors of the superficial zone cells. A gain\u2010 and loss\u2010of\u2010function strategy were used in an in vitro fluid flow shear stress (FFSS) model and an in vivo bilateral elevation bite model which showed mandibular condylar cartilage thickening. CaSR and PTHrP\n                    <jats:sub>87\u2010139<\/jats:sub>\n                    were modulated through treating the isolated superficial zone cells with activator\/SiRNA and via deleting CaSR or parathyroid hormone\u2010related peptide (PTHrP) gene in mice with the promoter gene of proteoglycan 4 (\n                    <jats:italic>Prg4<\/jats:italic>\n                    \u2010\n                    <jats:italic>Cre<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>ERT2<\/jats:italic>\n                    <\/jats:sup>\n                    ) in the tamoxifen\u2010inducible pattern with or without additional injection of Cinacalcet, the CaSR agonist, or PTHrP\n                    <jats:sub>87\u2010139<\/jats:sub>\n                    peptide. FFSS stimulated CaSR and PTHrP expression, and accelerated proliferation of the Prg4\u2010expressing superficial zone cells, in which process CaSR acted as an up\u2010streamer of PTHrP. Proteoglycan 4 specific knockout of CaSR or PTHrP reduced the cartilage thickness, suppressed the proliferation and early differentiation of the superficial zone cells, and inhibited cartilage thickening and matrix production promoted by bilateral elevation bite. Injections of CaSR agonist Cinacalcet could not improve the phenotype caused by PTHrP mutation. Injections of PTHrP\n                    <jats:sub>87\u2010139<\/jats:sub>\n                    peptide rescued the cartilage from knockout of CaSR gene. CaSR modulates proliferation of the superficial zone cells in mandibular condylar cartilage through activation of PTHrP nuclear localization sequence. Our data support the therapeutic target of CaSR in promoting PTHrP production in superficial zone cartilage.\n                  <\/jats:p>","DOI":"10.1096\/fj.202300037rr","type":"journal-article","created":{"date-parts":[[2023,7,13]],"date-time":"2023-07-13T11:38:08Z","timestamp":1689248288000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["<scp>CaSR<\/scp>\n                    modulates proliferation of the superficial zone cells in temporomandibular joint cartilage via the\n                    <scp>PTHrP<\/scp>\n                    nuclear localization sequence"],"prefix":"10.1096","volume":"37","author":[{"given":"Peng","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Oral Anatomy and Physiology, School of Stomatology The Fourth Military Medical University  Xi'an China"},{"name":"School of Stomatology Jiamusi University  Jiamusi 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University  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shibin","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Oral Anatomy and Physiology, School of Stomatology The Fourth Military Medical University  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Oral Anatomy and Physiology, School of Stomatology The Fourth Military Medical University  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuejiao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Oral Anatomy and Physiology, School of Stomatology The Fourth Military Medical University  Xi'an China"},{"name":"School of Stomatology Jiamusi University  Jiamusi China"},{"name":"Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases, Shanghai Stomatological Hospital Fudan University  Shanghai 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