{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T18:27:39Z","timestamp":1793125659686,"version":"4.5.0"},"reference-count":50,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,5,23]],"date-time":"2023-05-23T00:00:00Z","timestamp":1684800000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI132963"],"award-info":[{"award-number":["AI132963"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Immuno\u2010oncology (IO)\u2010based therapies such as checkpoint inhibitors, bi\u2010specific antibodies, and CAR\u2010T\u2010cell therapies have shown significant success in the treatment of several cancer indications. However, these therapies can result in the development of severe adverse events, including cytokine release syndrome (CRS). Currently, there is a paucity of in vivo models that can evaluate dose\u2010response relationships for both tumor control and CRS\u2010related safety issues. We tested an in vivo PBMC humanized mouse model to assess both treatment efficacy against specific tumors and the concurrent cytokine release profiles for individual human donors after treatment with a CD19xCD3 bispecific T\u2010cell engager (BiTE). Using this model, we evaluated tumor burden, T\u2010cell activation, and cytokine release in response to bispecific T\u2010cell\u2010engaging antibody in humanized mice generated with different PBMC donors. The results show that PBMC engrafted NOD\u2010\n                    <jats:italic>scid Il2rg<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>null<\/jats:italic>\n                    <\/jats:sup>\n                    mice lacking expression of mouse MHC class I and II (NSG\u2010MHC\u2010DKO mice) and implanted with a tumor xenograft predict both efficacy for tumor control by CD19xCD3 BiTE and stimulated cytokine release. Moreover, our findings indicate that this PBMC\u2010engrafted model captures variability among donors for tumor control and cytokine release following treatment. Tumor control and cytokine release were reproducible for the same PBMC donor in separate experiments. The PBMC humanized mouse model described here is a sensitive and reproducible platform that identifies specific patient\/cancer\/therapy combinations for treatment efficacy and development of complications.\n                  <\/jats:p>","DOI":"10.1096\/fj.202300040r","type":"journal-article","created":{"date-parts":[[2023,5,23]],"date-time":"2023-05-23T10:57:31Z","timestamp":1684839451000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Simultaneous evaluation of treatment efficacy and toxicity for bispecific T\u2010cell engager therapeutics in a humanized mouse model"],"prefix":"10.1096","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4226-7224","authenticated-orcid":false,"given":"Jiwon","family":"Yang","sequence":"first","affiliation":[{"name":"The Jackson Laboratory  Sacramento California 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