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We reported that 14\u20103\u20103\u03b2 interacts with FKBP12 and synaptopodin to maintain the structure of actin fibers in podocytes. However, the precise localization and differential role of 14\u20103\u20103 isoforms in kidneys are unclear. Herein, we showed that 14\u20103\u20103\u03b2 in glomeruli was restricted in podocytes, and 14\u20103\u20103\u03c3 in glomeruli was expressed in podocytes and mesangial cells. Although 14\u20103\u20103\u03b2 was dominantly co\u2010localized with FKBP12 in the foot processes, a part of 14\u20103\u20103\u03b2 was co\u2010localized with Par3 at the slit diaphragm. 14\u20103\u20103\u03b2 interacted with Par3, and FKBP12 bound to 14\u20103\u20103\u03b2 competitively with Par3. Deletion of 14\u20103\u20103\u03b2 enhanced the interaction of Par3 with Par6 in podocytes. Gene silencing for 14\u20103\u20103\u03b2 altered the structure of actin fibers and process formation. 14\u20103\u20103\u03b2 and synaptopodin expression was decreased in podocyte injury models. In contrast, 14\u20103\u20103\u03c3 in podocytes was expressed in the primary processes. 14\u20103\u20103\u03c3 interacted with vimentin but not with the actin\u2010associated proteins FKBP12 and synaptopodin. Gene silencing for 14\u20103\u20103\u03c3 altered the structure of vimentin fibers and process formation. 14\u20103\u20103\u03c3 and vimentin expression was increased in the early phase of podocyte injury models but was decreased in the late stage. Together, the localization of 14\u20103\u20103\u03b2 at actin cytoskeleton plays a role in maintaining the foot processes and the Par complex in podocytes. In contrast, 14\u20103\u20103\u03c3 at vimentin cytoskeleton is essential for maintaining primary processes.<\/jats:p>","DOI":"10.1096\/fj.202300865r","type":"journal-article","created":{"date-parts":[[2023,8,31]],"date-time":"2023-08-31T15:12:16Z","timestamp":1693494736000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["14\u20103\u20103 Proteins stabilize actin and vimentin filaments to maintain processes in renal glomerular podocyte"],"prefix":"10.1096","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-8207-8504","authenticated-orcid":false,"given":"Hidenori","family":"Yasuda","sequence":"first","affiliation":[{"name":"Department of Cell Biology, Kidney Research Center Niigata University Graduate School of Medical and Dental Sciences  Niigata 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