{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T01:24:09Z","timestamp":1790472249443,"version":"4.1.0"},"reference-count":57,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,12,16]],"date-time":"2023-12-16T00:00:00Z","timestamp":1702684800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2024,1,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The urinary bladder is supplied by a rich network of sensory and autonomic axons, commonly visualized by immunolabeling for neural markers. This approach demonstrates overall network patterning but is less suited to understanding the structure of individual motor and sensory terminals within these complex plexuses. There is a further limitation visualizing the lightly myelinated (A\u2010delta) class of sensory axons that provides the primary mechanosensory drive for initiation of voiding. Whereas most unmyelinated sensory axons can be revealed by immunolabeling for specific neuropeptides, to date no unique neural marker has been identified to immunohistochemically label myelinated visceral afferents. We aimed to establish a non\u2010surgical method to visualize and map myelinated afferents in the bladder in rats. We found that in rats, the adeno\u2010associated virus (AAV), AAV\u2010PHP.S, which shows a high tropism for the peripheral nervous system, primarily transduced myelinated dorsal root ganglion neurons, enabling us to identify the structure and regional distribution of myelinated (mechanosensory) axon endings within the muscle and lamina propria of the bladder. We further identified the projection of myelinated afferents within the pelvic nerve and lumbosacral spinal cord. A minority of noradrenergic and cholinergic neurons in pelvic ganglia were transduced, enabling visualization and regional mapping of both autonomic and sensory axon endings within the bladder. Our study identified a sparse labeling approach for investigating myelinated sensory and autonomic axon endings within the bladder and provides new insights into the nerve\u2010bladder interface.<\/jats:p>","DOI":"10.1096\/fj.202301113r","type":"journal-article","created":{"date-parts":[[2023,12,16]],"date-time":"2023-12-16T08:40:00Z","timestamp":1702716000000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["An adeno\u2010associated viral labeling approach to visualize the meso\u2010 and microanatomy of mechanosensory afferents and autonomic innervation of the rat urinary bladder"],"prefix":"10.1096","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9390-2457","authenticated-orcid":false,"given":"Nicole M.","family":"Wiedmann","sequence":"first","affiliation":[{"name":"Department of Anatomy and Physiology University of Melbourne  Parkville Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2997-1340","authenticated-orcid":false,"given":"John\u2010Paul","family":"Fuller\u2010Jackson","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Physiology University of Melbourne  Parkville Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8458-4159","authenticated-orcid":false,"given":"Peregrine B.","family":"Osborne","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Physiology University of Melbourne  Parkville Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4341-3265","authenticated-orcid":false,"given":"Janet R.","family":"Keast","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Physiology University of Melbourne  Parkville Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,12,16]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tins.2018.07.008"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-444-63247-0.00005-5"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(90)91693-b"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1994.72.5.2420"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.01049.2007"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-07898-y"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.2000.84.4.1924"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0189941"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2830-7"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/j.pain.0000000000002795"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnint.2018.00005"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2009.172577"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41583-021-00489-x"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2006.06.007"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2013.00206"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.23648"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1023\/a:1006903507321"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(96)12418-6"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00157961"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1523\/ENEURO.0397-19.2019"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-16499-6_19"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1426-20.2020"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autneu.2020.102697"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.24362"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.4593"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2023.1001007"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1109\/titb.2002.1006304"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"key":"e_1_2_11_30_1","volume-title":"R: A Language and Environment for Statistical Computing","author":"R Core Team","year":"2023"},{"key":"e_1_2_11_31_1","volume-title":"RStudio: Integrated Development Environment for R","author":"Posit Team","year":"2023"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0361-9230(93)90250-f"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.20-01-00427.2000"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41585-021-00428-6"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-physiol-020518-114525"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2017-315631"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordhb\/9780190860509.013.4"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2023.07.007"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0074-7696(06)48003-7"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00224723"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/0361-9230(92)90134-j"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/joa.13221"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1097\/j.pain.0000000000001453"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/1096-9861(20001218)428:3<558::AID-CNE11>3.0.CO;2-M"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004290050037"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2001.00255.x"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.24949"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.12-12-04878.1992"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.1993.265.2.R326"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.2000.279.1.R295"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1523\/ENEURO.0364-21.2021"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.20816"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2022.05.003"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.1177\/1744806917693003"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.12.010"},{"key":"e_1_2_11_56_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001190305"},{"key":"e_1_2_11_57_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1716.1975.tb05859.x"},{"key":"e_1_2_11_58_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902110102"}],"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.202301113R","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T16:42:12Z","timestamp":1761669732000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fj.202301113R"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,12,16]]},"references-count":57,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,1,15]]}},"alternative-id":["10.1096\/fj.202301113R"],"URL":"https:\/\/doi.org\/10.1096\/fj.202301113r","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"value":"0892-6638","type":"print"},{"value":"1530-6860","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,12,16]]},"assertion":[{"value":"2023-06-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-12-05","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-12-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e23380"}}