{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T10:45:31Z","timestamp":1789728331957,"version":"4.0.1"},"reference-count":36,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T00:00:00Z","timestamp":1690243200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009707","name":"Diabetesforbundet","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009707","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017245","name":"UNIFOR","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100017245","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2023,8]]},"abstract":"Abstract<\/jats:title>\n \n Nr1i2<\/jats:italic>\n , a nuclear receptor known for its key function in xenobiotic detoxification, has emerged as a potential regulator of intestinal homeostasis and inflammation. However, the role of\n Nr1i2<\/jats:italic>\n in different intestinal segments remains poorly known. Moreover, in vivo investigations on intestinal\n Nr1i2<\/jats:italic>\n have essentially been performed in whole\u2010body\n Nr1i2<\/jats:italic>\n knockout (\n Nr1i2<\/jats:italic>\n \u2212\/\u2212<\/jats:sup>\n ) mice where the deletion of\n Nr1i2<\/jats:italic>\n in all tissues may affect the intestinal phenotype. To better understand the role of\n Nr1i2<\/jats:italic>\n in the intestine, we generated intestinal epithelial\u2010specific\n Nr1i2<\/jats:italic>\n knockout (i\n Nr1i2<\/jats:italic>\n \u2212\/\u2212<\/jats:sup>\n ) mice and studied the duodenum, jejunum, ileum, and colon of these animals during steady\u2010state conditions and lipopolysaccharide (LPS)\u2010induced inflammation. As compared to control (i\n Nr1i2<\/jats:italic>\n +\/+<\/jats:sup>\n ) mice, i\n Nr1i2<\/jats:italic>\n \u2212\/\u2212<\/jats:sup>\n mice showed normal intestinal permeability as assessed by in vivo FITC\u2010dextran test. The expression of genes involved in inflammation, tight\u2010 and adherens\u2010junction, proliferation, glucose, and lipid metabolism was comparable in the duodenum, jejunum, ileum, and colon of i\n Nr1i2<\/jats:italic>\n \u2212\/\u2212<\/jats:sup>\n and i\n Nr1i2<\/jats:italic>\n +\/+<\/jats:sup>\n mice. In line with these findings, histological analyses of the jejunum revealed no difference between i\n Nr1i2<\/jats:italic>\n \u2212\/\u2212<\/jats:sup>\n and i\n Nr1i2<\/jats:italic>\n +\/+<\/jats:sup>\n mice. When treated with LPS, the intestine of i\n Nr1i2<\/jats:italic>\n \u2212\/\u2212<\/jats:sup>\n mice had no increased inflammatory response as compared to i\n Nr1i2<\/jats:italic>\n +\/+<\/jats:sup>\n mice. Moreover, the health monitoring of LPS\u2010treated i\n Nr1i2<\/jats:italic>\n \u2212\/\u2212<\/jats:sup>\n and i\n Nr1i2<\/jats:italic>\n +\/+<\/jats:sup>\n mice was similar. Taken together, our results demonstrate that the specific deletion of\n Nr1i2<\/jats:italic>\n in the intestinal epithelium does not cause major intestinal damages in mice during both steady\u2010state and inflammatory conditions.\n <\/jats:p>","DOI":"10.1096\/fj.202301126","type":"journal-article","created":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T11:32:22Z","timestamp":1690284742000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Mice lacking intestinal\n Nr1i2<\/i>\n have normal intestinal homeostasis under steady\u2010state conditions and are not hypersensitive to inflammation under lipopolysaccharide treatment"],"prefix":"10.1096","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9606-7810","authenticated-orcid":false,"given":"Salah Edden","family":"Amini","sequence":"first","affiliation":[{"name":"Department of Molecular Medicine, Institute of Basic Medical Sciences, Faculty of Medicine University of Oslo Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8623-2237","authenticated-orcid":false,"given":"Sophie Emilie","family":"Bresson","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine, Institute of Basic Medical Sciences, Faculty of Medicine University of Oslo Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3676-8145","authenticated-orcid":false,"given":"J\u00e9r\u00f4me","family":"Ruzzin","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine, Institute of Basic Medical Sciences, Faculty of Medicine University of Oslo Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,7,25]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1210\/er.2001-0038"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2017.07.846"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.12.20.3195"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI3703"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.051551698"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2014.06.014"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1515\/BC.2002.175"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1124\/pr.109.002014"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2002-30098"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.2337\/db12-1039"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-16104-4_9"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s15010-018-1178-5"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI124366"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.2337\/db06-1491"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI26283"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00528.2006"},{"key":"e_1_2_13_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0808146106"},{"key":"e_1_2_13_19_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201902083RR"},{"key":"e_1_2_13_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep31936"},{"key":"e_1_2_13_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0036075"},{"key":"e_1_2_13_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/gene.20042"},{"key":"e_1_2_13_23_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1241214"},{"key":"e_1_2_13_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756-0500-7-233"},{"key":"e_1_2_13_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep46252"},{"key":"e_1_2_13_26_1","volume-title":"Anatomy, 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