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The regulatory mechanisms and the functional importance of this process have been demonstrated in mice; however, regulation of this process in human and domestic animal is still largely unknown. In this study, we employed single\u2010cell RNA sequencing to identify transcriptional signatures of prospermatogonia and major somatic cell types in testes of goats at E85, E105, and E125. We identified both common and specific Gene Ontology categories, transcription factor regulatory networks, and cell\u2013cell interactions in cell types from goat testis. We also analyzed the transcriptional dynamic changes in prospermatogonia, Sertoli cells, Leydig cells, and interstitial cells. Our datasets provide a useful resource for the study of domestic animal germline development.<\/jats:p>","DOI":"10.1096\/fj.202301278","type":"journal-article","created":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T08:23:35Z","timestamp":1697099015000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Single\u2010cell transcriptome analysis of the germ cells and somatic cells during mitotic quiescence stage in goats"],"prefix":"10.1096","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6577-6705","authenticated-orcid":false,"given":"Min","family":"Chen","sequence":"first","affiliation":[{"name":"Guangdong and Shenzhen Key Laboratory of Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital Shenzhen Peking University\u2010The Hong Kong University of Science and Technology Medical Center  Shenzhen 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