{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T05:27:35Z","timestamp":1791523655299,"version":"4.3.4"},"reference-count":51,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2023,11,7]],"date-time":"2023-11-07T00:00:00Z","timestamp":1699315200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Spermatogonial stem cells (SSCs) play a crucial role in mammalian spermatogenesis and maintain the stable inheritance of the germline in livestock. However, stress and bacterial or viral infections can disrupt immune homeostasis of the testes, thereby leading to spermatogenesis destruction and infertility, which severely affects the health and productivity of mammals. This study aimed to explore the effect of ubiquitin C\u2010terminal hydrolase L1 (UCHL1) knockdown (KD) in goat SSCs and mouse testes and investigate the potential anti\u2010inflammatory function of UCHL1 in a poly(I:C)\u2010induced inflammation model to maintain microenvironmental homeostasis. In\u00a0vitro, the downregulation of UCHL1 (UCHL1 KD) in goat SSCs increased the expression levels of apoptosis and inflammatory factors and inhibited the self\u2010renewal and proliferation of SSCs. In\u00a0vivo, the structure of seminiferous tubules and spermatogenic cells was disrupted after UCHL1 KD, and the expression levels of apoptosis\u2010 and inflammation\u2010related proteins were significantly upregulated. Furthermore, UCHL1 inhibited the TLR3\/TBK1\/IRF3 pathway to resist poly(I:C)\u2010induced inflammation in SSCs by antagonizing HSPA8 and thus maintaining SSC autoimmune homeostasis. Most importantly, the results of this study showed that UCHL1 maintained immune homeostasis of SSCs and spermatogenesis. UCHL1 KD not only inhibited the self\u2010renewal and proliferation of goat SSCs and spermatogenesis but was also involved in the inflammatory response of goat SSCs. Additionally, UCHL1 has an antiviral function in SSCs by antagonizing HSPA8, which provides an important basis for exploring the specific mechanisms of UCHL1 in goat spermatogenesis.<\/jats:p>","DOI":"10.1096\/fj.202301674rr","type":"journal-article","created":{"date-parts":[[2023,11,7]],"date-time":"2023-11-07T09:17:07Z","timestamp":1699348627000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["<scp>UCHL1<\/scp>\n                    maintains microenvironmental homeostasis in goat germline stem cells"],"prefix":"10.1096","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1516-9875","authenticated-orcid":false,"given":"Donghui","family":"Yang","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering &amp; Technology Northwest A&amp;F University  Yangling 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