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Previous studies have shown that frozen embryo transfer is associated with an elevated risk of adverse maternal and neonatal outcomes. This study aimed to explore the effects of mouse blastocyst vitrification on the phenotype of vitrified\u2010warmed blastocysts, their intrauterine and postnatal development, and the long\u2010term metabolic health of the derived offspring. The vitrified\u2010warmed blastocysts (IVF\u2009+\u2009VT group) exhibited reduced mitochondrial activity, increased apoptotic levels, and decreased cell numbers when compared to the fresh blastocysts (IVF group). Implantation rates, live pup rates, and crown\u2010rump length at E18.5 were not different between the two groups. However, there was a significant decrease in fetal weight and fetal\/placental weight ratio in the IVF\u2009+\u2009VT group. 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