{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T19:04:45Z","timestamp":1767035085518,"version":"build-2065373602"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,12,15]],"date-time":"2023-12-15T00:00:00Z","timestamp":1702598400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81871181"],"award-info":[{"award-number":["81871181"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2024,1,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The uterine contraction during labor, a process with repetitive hypoxia and high energy consumption, is essential for successful delivery. However, the molecular mechanism of myometrial contraction regulation is unknown. Serpin family E member 1 (SERPINE1), one of the most upregulated genes in laboring myometrium in both transcriptome and proteome, was highlighted in our previous study. Here, we confirmed SERPINE1 is upregulated in myometrium during labor. Blockade of SERPINE1 using small interfering RNA (siRNA) or inhibitor (Tiplaxtinin) under hypoxic conditions in myocytes or myometrium in\u00a0vitro showed a decrease contractility, which was achieved by regulating ATP production. Chromatin immunoprecipitation (ChIP\u2010seq), Co\u2010immunoprecipitation (Co\u2010IP), and glutathione\u2010S\u2010transferase (GST) pull down explored that the promoter of SERPINE1 is directly activated by hypoxia\u2010inducible factor\u20101\u03b1 (HIF\u20101\u03b1) and SERPINE1 interacts with ATP Synthase Peripheral Stalk Subunit F6 (ATP5PF). Together they enhance hypoxia driven myometrial contraction by maintaining ATP production in the key oxidative phosphorylation pathway. The results provide new insight for uterine contraction regulation, and potential novel therapeutic targets for labor management.<\/jats:p>","DOI":"10.1096\/fj.202301804r","type":"journal-article","created":{"date-parts":[[2023,12,15]],"date-time":"2023-12-15T15:32:05Z","timestamp":1702654325000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Serpin family E member 1 enhances myometrium contractility by increasing\n                    <scp>ATP<\/scp>\n                    production during labor"],"prefix":"10.1096","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3158-1325","authenticated-orcid":false,"given":"Xiaodi","family":"Wang","sequence":"first","affiliation":[{"name":"Guangzhou Key Laboratory of Maternal\u2010Fetal Medicine, Department of Obstetrics and Gynecology Guangzhou Women and Children's Medical Center, Guangzhou Medical University  Guangzhou PR 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