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However, the metabolic mechanism of IL\u201025\u2010induced tuft cell expansion is largely unknown. Folate metabolism provides essential purine and methyl substrates for cell proliferation and differentiation. Thus, we aim to investigate the roles of folate metabolism playing in IL\u201025\u2010induced tuft cell expansion by enteroviral infection and recombinant murine IL\u201025 (rmIL\u201025) protein\u2010stimulated mouse models. At present, enteroviruses, such as EV71, CVA16, CVB3, and CVB4, upregulated IL\u201025 expression and induced tuft cell expansion in the intestinal tissues of mice. However, EV71 did not induce intestinal tuft cell expansion in\n                    <jats:italic>IL\u201025<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice. Interestingly, compared to the mock group, folate was enriched in the intestinal tissues of both the EV71\u2010infected group and the rmIL\u201025 protein\u2010stimulated group. Moreover, folate metabolism supported IL\u201025\u2010induced tuft cell expansion since both folate\u2010depletion and anti\u2010folate MTX\u2010treated mice had a disrupted tuft cell expansion in response to rmIL\u201025 protein stimulation. In summary, our data suggested that folate metabolism supported intestinal tuft cell expansion in response to enterovirus\u2010induced IL\u201025 expression, which provided a new insight into the mechanisms of tuft cell expansion from the perspective of folate metabolism.\n                  <\/jats:p>","DOI":"10.1096\/fj.202301928r","type":"journal-article","created":{"date-parts":[[2024,1,20]],"date-time":"2024-01-20T04:49:25Z","timestamp":1705726165000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Folate supports\n                    <scp>IL<\/scp>\n                    \u201025\u2010induced tuft cell expansion following enteroviral infections"],"prefix":"10.1096","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7473-2181","authenticated-orcid":false,"given":"Ruining","family":"Lyu","sequence":"first","affiliation":[{"name":"Center for Public Health Research, Medical School Nanjing University  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