{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T07:56:10Z","timestamp":1791618970221,"version":"4.3.4"},"reference-count":54,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,12,5]],"date-time":"2023-12-05T00:00:00Z","timestamp":1701734400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["K23DK109263"],"award-info":[{"award-number":["K23DK109263"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2024,1,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>MicroRNA (miRNA)\u2010mediated mRNA regulation directs many homeostatic and pathological processes, but how miRNAs coordinate aberrant esophageal inflammation during eosinophilic esophagitis (EoE) is poorly understood. Here, we report a deregulatory axis where microRNA\u2010155 (miR\u2010155) regulates epithelial barrier dysfunction by selectively constraining tight junction CLDN7 (claudin\u20107). MiR\u2010155 is elevated in the esophageal epithelium of biopsies from patients with active EoE and in cell culture models. MiR\u2010155 localization using in\u00a0situ hybridization (ISH) in patient biopsies and intra\u2010epithelial compartmentalization of miR\u2010155 show expression predominantly within the basal epithelia. Epithelial miR\u2010155 activity was evident through diminished target gene expression in 3D organotypic cultures, particularly in relatively undifferentiated basal cell states. Mechanistically, generation of a novel cell line with enhanced epithelial miR\u2010155 stable overexpression induced a functionally deficient epithelial barrier in 3D air\u2013liquid interface epithelial cultures measured by transepithelial electrical resistance (TEER). Histological assessment of 3D esophageal organoid cultures overexpressing miR\u2010155 showed notable dilated intra\u2010epithelial spaces. Unbiased RNA\u2010sequencing analysis and immunofluorescence determined a defect in epithelial barrier tight junctions and revealed a selective reduction in the expression of critical esophageal tight junction molecule, claudin\u20107. Together, our data reveal a previously unappreciated role for miR\u2010155 in mediating epithelial barrier dysfunction in esophageal inflammation.<\/jats:p>","DOI":"10.1096\/fj.202301934r","type":"journal-article","created":{"date-parts":[[2023,12,5]],"date-time":"2023-12-05T03:46:35Z","timestamp":1701747995000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Hypoxia\u2010inducible\n                    <scp>microRNA<\/scp>\n                    \u2010155 negatively regulates epithelial barrier in eosinophilic esophagitis by suppressing tight junction claudin\u20107"],"prefix":"10.1096","volume":"38","author":[{"given":"Gary E.","family":"Markey","sequence":"first","affiliation":[{"name":"Allergy, Inflammation &amp; Remodelling Research Laboratory, Kathleen Lonsdale Institute for Human Health Research, Department of Biology National University of Ireland  Maynooth Co. Kildare Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sin\u00e9ad","family":"Ryan","sequence":"additional","affiliation":[{"name":"Allergy, Inflammation &amp; Remodelling Research Laboratory, Kathleen Lonsdale Institute for Human Health Research, Department of Biology National University of Ireland  Maynooth Co. Kildare Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Glenn T.","family":"Furuta","sequence":"additional","affiliation":[{"name":"Gastrointestinal Eosinophilic Diseases Program, Digestive Health Institute, Children's Hospital Colorado, Department of Pediatrics University of Colorado School of Medicine  Aurora Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Calies","family":"Menard\u2010Katcher","sequence":"additional","affiliation":[{"name":"Gastrointestinal Eosinophilic Diseases Program, Digestive Health Institute, Children's Hospital Colorado, Department of Pediatrics University of Colorado School of Medicine  Aurora Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eoin N.","family":"McNamee","sequence":"additional","affiliation":[{"name":"Mucosal Immunology Research Laboratory, Kathleen Lonsdale Institute for Human Health Research, Department of Biology National University of Ireland  Maynooth Co. Kildare Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8336-9879","authenticated-orcid":false,"given":"Joanne C.","family":"Masterson","sequence":"additional","affiliation":[{"name":"Allergy, Inflammation &amp; Remodelling Research Laboratory, Kathleen Lonsdale Institute for Human Health Research, Department of Biology National University of Ireland  Maynooth Co. Kildare Ireland"},{"name":"Gastrointestinal Eosinophilic Diseases Program, Digestive Health Institute, Children's Hospital Colorado, Department of Pediatrics University of Colorado School of Medicine  Aurora Colorado 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