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We transferred\n                    <jats:italic>Bos taurus taurus<\/jats:italic>\n                    embryos of different origin, in\u00a0vivo (high developmental potential (IV)), in\u00a0vitro (intermediate developmental potential (IVF)), or cloned (low developmental potential (NT)), into\n                    <jats:italic>Bos taurus indicus<\/jats:italic>\n                    recipients. Extracellular vesicles (EVs) recovered from Day 16 conceptus\u2010conditioned medium were characterized and their microRNA (miRNA) cargo sequenced alongside RNA sequencing of their respective endometria. There were substantial differences in the endometrial response to in\u00a0vivo versus in\u00a0vitro and in\u00a0vivo versus cloned conceptuses (1153 and 334DEGs respectively) with limited differences between in\u00a0vitro Vs cloned conceptuses (36 DEGs). The miRNA cargo contained in conceptus\u2010derived EVs was similar between all three groups (426 miRNA in common). Only 8 miRNAs were different between in\u00a0vivo and cloned conceptuses, while only 6 miRNAs were different between in\u00a0vivo and in\u00a0vitro\u2010derived conceptuses. Treatment of endometrial epithelial cells with mimic or inhibitors for miR\u2010128 and miR\u20101298 changed the proteomic content of target cells (96 and 85, respectively) of which mRNAs are altered in the endometrium in\u00a0vivo (\n                    <jats:italic>PLXDC2, COPG1, HSPA12A, MCM5, TBL1XR1<\/jats:italic>\n                    , and\n                    <jats:italic>TTF<\/jats:italic>\n                    )\n                    <jats:italic>.<\/jats:italic>\n                    In conclusion, we have determined that the biosensor capability of the endometrium is mediated in part, by its response to different EVs miRNA cargo produced by the conceptus during the peri\u2010implantation period of pregnancy.\n                  <\/jats:p>","DOI":"10.1096\/fj.202302423rr","type":"journal-article","created":{"date-parts":[[2024,5,14]],"date-time":"2024-05-14T08:09:41Z","timestamp":1715674181000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Biosensor capability of the endometrium is mediated in part, by altered miRNA cargo from conceptus\u2010derived extracellular vesicles"],"prefix":"10.1096","volume":"38","author":[{"given":"Tiago H. 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