{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T22:55:34Z","timestamp":1793660134973,"version":"4.5.1"},"reference-count":58,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2024,9,6]],"date-time":"2024-09-06T00:00:00Z","timestamp":1725580800000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2024,9,1]],"date-time":"2024-09-01T00:00:00Z","timestamp":1725148800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Pre\u2010implantation embryonic development occurs in the oviduct during the first few days of pregnancy. The presence of oviductal extracellular vesicles (oEVs, also called oviductosomes) is crucial for pre\u2010implantation embryonic development in\u00a0vivo as oEVs often contain molecular transmitters such as proteins. Therefore, evaluating oEV cargo during early pregnancy could provide insights into factors required for proper early embryonic development that are missing in the current in\u00a0vitro embryo culture setting. In this study, we isolated oEVs from the oviductal fluid at estrus and different stages of early embryonic development. The 2306\u20133066 proteins in oEVs identified at the different time points revealed 58\u201360 common EV markers identified in exosome databases. Oviductal extracellular vesicle proteins from pregnant samples significantly differed from those in non\u2010pregnant samples. In addition, superovulation changes the protein contents in oEVs compared to natural ovulation at estrus. Importantly, we have identified that embryo\u2010protectant proteins such as high\u2010mobility protein group B1 and serine (or cysteine) peptidase inhibitor were only enriched in the presence of embryos. We also visualized the physical interaction of EVs and the zona pellucida of 4\u2010 to 8\u2010cell stage embryos using transmission electron microscopy as well as in\u00a0vivo live imaging of epithelial cell\u2010derived GFP\u2010tagged CD9 mouse model. All protein data in this study are readily available to the scientific community in a searchable format at\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"https:\/\/genes.winuthayanon.com\/winuthayanon\/oviduct_ev_proteins\/\">https:\/\/genes.winuthayanon.com\/winuthayanon\/oviduct_ev_proteins\/<\/jats:ext-link>\n                    . In conclusion, we identified oEVs proteins that could be tested to determine whether they can improve embryonic developmental outcomes in\u00a0vivo and in\u00a0vitro setting.\n                  <\/jats:p>","DOI":"10.1096\/fj.202400041rr","type":"journal-article","created":{"date-parts":[[2024,9,6]],"date-time":"2024-09-06T06:35:09Z","timestamp":1725604509000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Proteomic analysis and in\u00a0vivo visualization of extracellular vesicles from mouse oviducts during pre\u2010implantation embryo development"],"prefix":"10.1096","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9323-3764","authenticated-orcid":false,"given":"Kalli K.","family":"Stephens","sequence":"first","affiliation":[{"name":"Division of Animal Sciences, College of Agriculture, Food and Natural Resources University of Missouri  Columbia Missouri 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