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Lipidomics analysis of brains at 2, 7, and 18\u2009months of age showed that phosphatidylcholines, which make up about two\u2010thirds of all cerebrum membrane lipids, contain a gross excess of saturated fatty acids in AdipoR2 knockout mice, and that this is mostly attributed to an excess palmitic acid (C16:0) at the expense of oleic acid (C18:1), consistent with a defect in fatty acid desaturation and elongation in the mutant. Specifically, there was a\u2009~12% increase in the overall saturated fatty acid content within phosphatidylcholines and a\u2009~30% increase in phosphatidylcholines containing two palmitic acids. Phosphatidylethanolamines, sphingomyelins, ceramides, lactosylceramides, and dihydroceramides also showed an excess of saturated fatty acids in the AdipoR2 knockout mice while nervonic acid (C24:1) was enriched at the expense of shorter saturated fatty acids in glyceroceramides. Similar defects were found in the cerebellum and myelin sheaths. Histology showed that cell density is lower in the cerebrum of AdipoR2 knockout mice, but electron microscopy did not detect reproducible defects in the ultrastructure of cerebrum neurons, though proteomics analysis showed an enrichment of electron transport chain proteins in the cerebellum. Behavioral tests showed that older (33\u2009weeks old) AdipoR2 knockout mice are hyperactive and anxious compared to control mice of a similar age. Also, in contrast to control mice, the AdipoR2 knockout mice do not gain weight in old age but do have normal lifespans. We conclude that an excess fatty acid saturation in brain phospholipids is accompanied by hyperactivity but seems otherwise well tolerated.<\/jats:p>","DOI":"10.1096\/fj.202400293rr","type":"journal-article","created":{"date-parts":[[2024,7,11]],"date-time":"2024-07-11T04:59:08Z","timestamp":1720673948000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Aging\n                    <scp>AdipoR2<\/scp>\n                    \u2010deficient mice are hyperactive with enlarged brains excessively rich in saturated fatty acids"],"prefix":"10.1096","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7149-6600","authenticated-orcid":false,"given":"Mario","family":"Ruiz","sequence":"first","affiliation":[{"name":"Department of Chemistry and Molecular Biology University of Gothenburg  Gothenburg 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