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The aim of the present study is to investigate gut dysbiosis in this progression. Fecal microbial rRNA\u201016S sequencing, absolute quantification, histopathologic, and biochemical tests were performed in mice fed high fat\/calorie diet plus high fructose and glucose in drinking water (HFCD\u2010HF\/G) or control diet (CD) for 2, 16\u2009weeks, or 14\u2009months. Histopathologic examination verified an early stage of SFL, MASH, fibrotic, or MASH\u2010HCC progression with disturbance of lipid metabolism, liver injury, and impaired gut mucosal barrier as indicated by loss of occludin in ileum mucosa. Gut dysbiosis occurred as early as 2\u2009weeks with reduced \u03b1 diversity, expansion of\n                    <jats:italic>Kineothrix<\/jats:italic>\n                    ,\n                    <jats:italic>Lactococcus<\/jats:italic>\n                    ,\n                    <jats:italic>Akkermansia<\/jats:italic>\n                    ; and shrinkage in\n                    <jats:italic>Bifidobacterium<\/jats:italic>\n                    ,\n                    <jats:italic>Lactobacillus<\/jats:italic>\n                    , etc., at a genus level. Dysbiosis was found as early as MAHS initiation, and was much more profound through the MASH\u2010fibrotic and oncogenic progression. Moreover, the expansion of specific species, such as\n                    <jats:italic>Lactobacillus johnsonii<\/jats:italic>\n                    and\n                    <jats:italic>Kineothrix alysoides<\/jats:italic>\n                    , was confirmed by an optimized method for absolute quantification. Dynamic alterations of gut microbiota were characterized in three stages of early SFL, MASH, and its HCC transformation. The findings suggest that the extent of dysbiosis was accompanied with MASH progression and its transformation to HCC, and the shrinking or emerging of specific microbial species may account at least in part for pathologic, metabolic, and immunologic alterations in fibrogenic progression and malignant transition in the liver.\n                  <\/jats:p>","DOI":"10.1096\/fj.202400573rr","type":"journal-article","created":{"date-parts":[[2024,7,5]],"date-time":"2024-07-05T06:50:35Z","timestamp":1720162235000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Dynamic changes of gut microbiota in mouse models of metabolic dysfunction\u2010associated steatohepatitis and its transition to hepatocellular carcinoma"],"prefix":"10.1096","volume":"38","author":[{"given":"Yu\u2010Li","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology and Parasitology, MOE\/NHC\/CAMS Key Laboratory of 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