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Localization of Calpain 3 within the triad suggests it contributes to Ca\n                    <jats:sup>2+<\/jats:sup>\n                    homeostasis. Through live\u2010cell Ca\n                    <jats:sup>2+<\/jats:sup>\n                    measurements, muscle mechanics, immunofluorescence, and electron microscopy (EM) in\n                    <jats:italic>Capn3<\/jats:italic>\n                    deficient (C3KO) and wild\u2010type (WT) mice, we determined whether loss of Calpain 3 altered Store\u2010Operated Calcium Entry (SOCE) activity. Direct Ca\n                    <jats:sup>2+<\/jats:sup>\n                    influx measurements revealed loss of\n                    <jats:italic>Capn3<\/jats:italic>\n                    elicits elevated resting SOCE and increased resting cytosolic Ca\n                    <jats:sup>2+<\/jats:sup>\n                    , supported by high incidence of calcium entry units (CEUs) observed by EM. C3KO and WT mice were subjected to a single bout of treadmill running to elicit SOCE. Within 1HR post\u2010treadmill running, C3KO mice exhibited diminished force production in\n                    <jats:italic>extensor digitorum longus<\/jats:italic>\n                    muscles and a greater decay of Ca\n                    <jats:sup>2+<\/jats:sup>\n                    transients in\n                    <jats:italic>flexor digitorum brevis<\/jats:italic>\n                    muscle fibers during repetitive stimulation. Striking evidence for impaired exercise\u2010induced SOCE activation in C3KO mice included poor colocalization of key SOCE proteins, stromal\u2010interacting molecule 1 (STIM1) and ORAI1, combined with disappearance of CEUs in C3KO muscles. These results demonstrate that Calpain 3 is a key regulator of SOCE in skeletal muscle and identify SOCE dysregulation as a contributing factor to LGMD R1\/2A pathology.\n                  <\/jats:p>","DOI":"10.1096\/fj.202400697r","type":"journal-article","created":{"date-parts":[[2024,7,19]],"date-time":"2024-07-19T11:48:24Z","timestamp":1721389704000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Loss of Calpain 3 dysregulates store\u2010operated calcium entry and its exercise response in mice"],"prefix":"10.1096","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-3274-0923","authenticated-orcid":false,"given":"Katelyn R.","family":"Villani","sequence":"first","affiliation":[{"name":"Department of Applied Physiology and Kinesiology, College of Health and Human Performance University of Florida  Gainesville Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4448-6551","authenticated-orcid":false,"given":"Renjia","family":"Zhong","sequence":"additional","affiliation":[{"name":"Department of Applied Physiology and Kinesiology, College of Health and Human Performance University of Florida  Gainesville Florida USA"},{"name":"Department of Emergency Medicine the First Affiliated Hospital of China Medical University  Shenyang Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-3839-6332","authenticated-orcid":false,"given":"C. 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