{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T18:30:32Z","timestamp":1787596232904,"version":"build-2736575974"},"reference-count":44,"publisher":"Wiley","issue":"23","license":[{"start":{"date-parts":[[2024,11,30]],"date-time":"2024-11-30T00:00:00Z","timestamp":1732924800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2024,12,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>As a common digestive disease, acute pancreatitis (AP) often threatens the life of patients. Bone marrow mesenchymal stem cells (BMSCs) derived exosomes have exhibited some benefits for AP. However, the mechanism remains unclear and deserves to be further investigated. The characteristics of BMSCs\u2010exosomes (BMSCs\u2010Exos) were identified. The abundance of genes and proteins was evaluated using quantitative real\u2010time PCR (RT\u2010qPCR), western blot, enzyme\u2010linked immunosorbent assay (ELISA) and IF assay. Cell apoptosis and CD206\u2010positive cells were measured by flow cytometry. The interactions among miR\u2010181a\u20105p, Zinc finger E\u2010box binding homeobox\u00a02 (ZEB2) and Receptor for Activated C Kinase 1 (RACK1) were verified using dual luciferase reporter assay, RNA immunoprecipitation (RIP), coimmunoprecipitation (Co\u2010IP). BMSCs\u2010Exos effectively improved AP injury through restraining AR42J cell apoptosis and promoting M2 macrophage polarization, which was realized due to BMSCs\u2010Exos harboring an abundance of miR\u2010181a\u20105p. Further experiments validated miR\u2010181a\u20105p silenced ZEB2 and ZEB2 reduced RACK1 expression through mediating RACK1 ubiquitination. ZEB2 knockdown decreased AR42J cell apoptosis and induced M2 macrophage polarization to alleviate AP injury, whereas RACK1 downregulation abolished these phenomena. BMSCs\u2010Exos harboring miR\u2010181a\u20105p suppressed AR42J cell apoptosis and promoted M2 macrophage polarization to delay AP progression through ZEB2\u2010mediated RACK1 ubiquitination.<\/jats:p>","DOI":"10.1096\/fj.202400803rr","type":"journal-article","created":{"date-parts":[[2024,11,30]],"date-time":"2024-11-30T03:20:32Z","timestamp":1732936832000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Bone marrow mesenchymal stem cell\u2010derived exosomal\n                    <scp>miR<\/scp>\n                    \u2010181a\u20105p promotes\n                    <scp>M2<\/scp>\n                    macrophage polarization to alleviate acute pancreatitis through\n                    <scp>ZEB2<\/scp>\n                    \u2010mediated\n                    <scp>RACK1<\/scp>\n                    ubiquitination"],"prefix":"10.1096","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-7855-8939","authenticated-orcid":false,"given":"Hanyu","family":"Li","sequence":"first","affiliation":[{"name":"The Second Department of General Surgery Yunnan University Affiliated Hospital  Kunming Yunnan P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-1803-1253","authenticated-orcid":false,"given":"Ruifeng","family":"Du","sequence":"additional","affiliation":[{"name":"The Second Department of General Surgery Yunnan University Affiliated Hospital  Kunming Yunnan P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-9380-3508","authenticated-orcid":false,"given":"Andong","family":"Xiang","sequence":"additional","affiliation":[{"name":"The Second Department of General Surgery Yunnan University Affiliated Hospital  Kunming Yunnan P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-8964-6104","authenticated-orcid":false,"given":"Yankui","family":"Liu","sequence":"additional","affiliation":[{"name":"The Second Department of General Surgery Yunnan University Affiliated Hospital  Kunming Yunnan P.R. 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