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To investigate the association between YAP and cataract, various experimental techniques were employed, including cell viability assessment, Annexin V FITC\/PI assay, measurement of ROS production, RT\u2010PCR, Western blot assay, and Immunoprecipitation. UVB exposure on human lens epithelium cells (HLECs) reduced total and nuclear YAP protein expression, increased cleaved\/pro\u2010caspase 3 ratios, decreased cell viability, and elevated ROS levels compared to controls. Similar Western blot results were observed in in\u00a0vivo experiments involving UVB\u2010treated mice. YAP knockdown in\u00a0vitro demonstrated a decrease in the protein expression of FOXM1, Nrf2, and HO\u20101, which correlated with the mRNA expression, accompanied by an increase in cell apoptosis, caspase 3 activation, and the release of ROS. Conversely, YAP overexpression mitigated these effects induced by UVB irradiation. Immunoprecipitation revealed a FOXM1\u2010YAP interaction. Notably, inhibiting FOXM1 decreased Nrf2 and HO\u20101, activating caspase 3. Additionally, administering the ROS inhibitor N\u2010acetyl\u2010L\u2010cysteine (NAC) effectively mitigated the apoptotic effects induced by oxidative stress from UVB irradiation, rescuing the protein expression levels of YAP, FOXM1, Nrf2, and HO\u20101. The initial findings of our study demonstrate the existence of a feedback loop involving YAP, FOXM1, Nrf2, and ROS that significantly influences the cell apoptosis in HLECs under UVB\u2010induced oxidative stress.<\/jats:p>","DOI":"10.1096\/fj.202400848r","type":"journal-article","created":{"date-parts":[[2024,7,24]],"date-time":"2024-07-24T10:23:13Z","timestamp":1721816593000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["A role for\n                    <scp>YAP<\/scp>\n                    \/\n                    <scp>FOXM1<\/scp>\n                    \/Nrf2 axis in oxidative stress and apoptosis of cataract induced by\n                    <scp>UVB<\/scp>\n                    irradiation"],"prefix":"10.1096","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-4767-8254","authenticated-orcid":false,"given":"Yingying","family":"Hong","sequence":"first","affiliation":[{"name":"Eye Institute and Department of Ophthalmology, Eye &amp; 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