{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T16:08:41Z","timestamp":1789661321184,"version":"4.0.1"},"reference-count":55,"publisher":"Wiley","issue":"22","license":[{"start":{"date-parts":[[2024,11,19]],"date-time":"2024-11-19T00:00:00Z","timestamp":1731974400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFD1800104"],"award-info":[{"award-number":["2021YFD1800104"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U20A2059"],"award-info":[{"award-number":["U20A2059"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2024,11,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>African swine fever virus (ASFV) is a large, icosahedral, double\u2010stranded DNA virus in the Asfarviridae family and the causative agent of African swine fever (ASF). ASFV causes a hemorrhagic fever with high mortality rates in domestic and wild pigs. ASFV contains an open reading frame named EP152R, previous research has shown that EP152R is an essential gene for virus rescue in swine macrophages. However, the detailed functions of ASFV EP152R remain elusive. Herein, we demonstrate that EP152R, a membrane protein located in the endoplasmic reticulum (ER), induces ER stress and swelling, triggering the PERK\/eIF2\u03b1 pathway, and broadly inhibiting host protein synthesis in\u00a0vitro. Additionally, EP152R strongly promotes immune evasion, reduces cell proliferation, and alters cellular metabolism. These results suggest that ASFV EP152R plays a critical role in the intracellular environment, facilitating viral replication. Furthermore, virus\u2010level experiments have shown that the knockdown of EP152R or PERK inhibitors efficiently affects viral replication by decreasing viral gene expression. In summary, these findings reveal a series of novel functions of ASFV EP152R and have important implications for understanding host\u2010pathogen interactions.<\/jats:p>","DOI":"10.1096\/fj.202400931rr","type":"journal-article","created":{"date-parts":[[2024,11,19]],"date-time":"2024-11-19T06:31:54Z","timestamp":1731997914000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["<scp>EP152R<\/scp>\n                    \u2010mediated endoplasmic reticulum stress contributes to African swine fever virus infection via the\n                    <scp>PERK<\/scp>\n                    \u2010\n                    <scp>eIF2\u03b1<\/scp>\n                    pathway"],"prefix":"10.1096","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2259-0112","authenticated-orcid":false,"given":"Rui","family":"Liang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Agricultural Microbiology, College of 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