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Our data have shown that KATNB1 expressed in rat brain, testes, and Sertoli cells. KATNB1 was found to co\u2010localize with \u03b1\u2010tubulin showing a unique stage\u2010specific distribution across the seminiferous epithelium. Knockdown of KATNB1 by RNAi led to significant disruption of the tight junction (TJ) permeability barrier function in primary Sertoli cells cultured in\u00a0vitro with an established functional TJ\u2010barrier, as well as perturbations in the microtubule and actin cytoskeleton organization. The disruption in these cytoskeletal structures, in turn, led to improper distribution of TJ and basal ES proteins essential for maintaining the Sertoli TJ function. More importantly, overexpression of KATNB1 in the testis in\u00a0vivo was found to block cadmium\u2010induced blood\u2013testis barrier (BTB) disruption and testis injury. KATNB1 exerted its promoting effects on BTB and spermatogenesis through corrective spatiotemporal expression of actin\u2010 and microtubule\u2010based regulatory proteins by maintaining the proper organization of cytoskeletons in the testis, illustrating its plausible therapeutic implication. In summary, Katanin regulatory subunit B1 (KATNB1)\u00a0plays a crucial role in BTB and spermatogenesis through its effects on the actin\u2010 and microtubule\u2010based cytoskeletons in Sertoli cells and testis, providing important insights into male reproductive biology.<\/jats:p>","DOI":"10.1096\/fj.202400966r","type":"journal-article","created":{"date-parts":[[2024,9,14]],"date-time":"2024-09-14T04:35:34Z","timestamp":1726288534000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Katanin regulatory subunit\n                    <scp>B1<\/scp>\n                    (\n                    <scp>KATNB1<\/scp>\n                    ) regulates\n                    <scp>BTB<\/scp>\n                    dynamics through changes in cytoskeletal organization"],"prefix":"10.1096","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-5679-5422","authenticated-orcid":false,"given":"Bai\u2010Ping","family":"Mao","sequence":"first","affiliation":[{"name":"Department of Anesthesiology and Perioperative Medicine The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University  Wenzhou China"},{"name":"Key Laboratory of Pediatric Anesthesiology Ministry of Education, Wenzhou Medical University  Wenzhou China"},{"name":"Key Laboratory of Anesthesiology of Zhejiang Province Wenzhou Medical University  Wenzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-2340-9434","authenticated-orcid":false,"given":"Mingdong","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Perioperative Medicine The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University  Wenzhou China"},{"name":"Key Laboratory of Pediatric Anesthesiology Ministry of Education, Wenzhou Medical University  Wenzhou China"},{"name":"Key Laboratory of Anesthesiology of Zhejiang Province Wenzhou Medical University  Wenzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2792-2761","authenticated-orcid":false,"given":"Yuanyuan","family":"Shan","sequence":"additional","affiliation":[{"name":"Department of Pathology The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University  Wenzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-7317-605X","authenticated-orcid":false,"given":"Ya\u2010Nan","family":"Wang","sequence":"additional","affiliation":[{"name":"Rehabilitation Medicine Center The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University  Wenzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6956-8452","authenticated-orcid":false,"given":"Huitao","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Perioperative Medicine The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University  Wenzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-9610-2500","authenticated-orcid":false,"given":"Jinhan","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Perioperative Medicine The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University  Wenzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-8534-8310","authenticated-orcid":false,"given":"Xuanjing","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Perioperative Medicine The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University  Wenzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4451-4479","authenticated-orcid":false,"given":"Ende","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Perioperative Medicine The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University  Wenzhou China"},{"name":"Key Laboratory of Pediatric Anesthesiology Ministry of Education, Wenzhou Medical University  Wenzhou China"},{"name":"Key Laboratory of Anesthesiology of Zhejiang Province Wenzhou Medical University  Wenzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. 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