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Several pyroptosis\u2010related factors are regulated by the centrosome. Pericentriolar material 1 (PCM1) is a primary component of centriolar satellites that is present as cytoplasmic granules around the centrosome. Our previous study revealed that PCM1 was highly expressed in UC patients, but the role of PCM1 in UC remains unknown. This study aimed to elucidate the role of PCM1 in the development of UC, especially the mechanism in pyroptosis process of UC. Clinical mucosal sample and dextran sulfate sodium (DSS)\u2010induced colitis mouse were used to reveal the association between PCM1 and intestinal inflammation. Intestinal epithelial cell\u2010specific PCM1\u2010knockout mice were constructed to determine the role of PCM1 in colitis. Finally, PCM1 RNA interference and overexpression assays in THP1 cells were employed to study the molecular mechanisms of PCM1 in inflammatory responses and pyroptosis. We found that PCM1 expression was upregulated in the colonic mucosa of UC patients and positively correlated with inflammatory indicators. PCM1 expression was elevated in DSS\u2010induced colitis mice and was reduced after methylprednisolone treatment. In the DSS colitis model, intestinal\u2010specific PCM1\u2010knockout mice exhibited milder intestinal inflammation and lower pyroptosis levels than wild\u2010type mice. In cell level, PCM1 exerted a proinflammatory effect by activating the NLRP3 inflammasome and triggering subsequent gasdermin D\u2010mediated pyroptosis to release IL\u20101\u03b2 and IL\u201018. In conclusion, PCM1 mediates activation of the NLRP3 inflammasome and gasdermin D\u2010dependent pyroptosis, ultimately accelerating intestinal inflammation in UC. These findings revealed a previously unknown role of PCM1 in initiating intestinal mucosal inflammation and pyroptosis in UC, and this factor is expected to be a regulator in the complex inflammatory network of UC.<\/jats:p>","DOI":"10.1096\/fj.202401185r","type":"journal-article","created":{"date-parts":[[2024,9,19]],"date-time":"2024-09-19T10:10:50Z","timestamp":1726740650000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Pericentriolar material 1 promotes intestinal inflammation in ulcerative colitis by activating\n                    <scp>NLRP3<\/scp>\n                    \/gasdermin D\u2010mediated macrophage pyroptosis"],"prefix":"10.1096","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9458-0138","authenticated-orcid":false,"given":"Junkun","family":"Niu","sequence":"first","affiliation":[{"name":"Department of Gastroenterology The First Affiliated Hospital of Kunming Medical University  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