{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T18:45:40Z","timestamp":1793040340076,"version":"4.5.0"},"reference-count":37,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2024,11,13]],"date-time":"2024-11-13T00:00:00Z","timestamp":1731456000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005153","name":"China National Funds for Distinguished Young Scientists","doi-asserted-by":"publisher","award":["32125035"],"award-info":[{"award-number":["32125035"]}],"id":[{"id":"10.13039\/501100005153","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFD1300200"],"award-info":[{"award-number":["2023YFD1300200"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2024,11,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    H3K27 acetylation (H3K27ac) is crucial in muscle development as it regulates gene expression. Dysregulation of H3K27ac level has been linked to muscle\u2010related diseases such as Duchenne muscular dystrophy, yet the mechanisms through which H3K27ac influences myogenic differentiation are not fully understood. Here, we utilized the SGC\u2010CBP30 drug, a CBP\/p300 bromodomain inhibitor, to reduce H3K27ac level and investigated its effect on myogenic differentiation of porcine skeletal muscle satellite cells. The results demonstrated an increased H3K27ac level during normal muscle satellite cell differentiation. We found that the addition of SGC\u2010CBP30 resulted in a reduced level of H3K27ac based on ATAC\u2010seq and CUT&amp;Tag data. Our analysis revealed that a cluster characterized by reduced levels of H3K27ac and increased levels of H3K27me3 was enriched with motifs corresponding to\n                    <jats:italic>Bach2<\/jats:italic>\n                    ,\n                    <jats:italic>MafK<\/jats:italic>\n                    , and\n                    <jats:italic>Fosl2<\/jats:italic>\n                    transcription factors. Furthermore, knockdown of\n                    <jats:italic>Bach2<\/jats:italic>\n                    ,\n                    <jats:italic>MafK<\/jats:italic>\n                    , and\n                    <jats:italic>Fosl2<\/jats:italic>\n                    produced a similar suppression effect on myogenic differentiation. Taken together, our study contributes to a better understanding of how H3K27ac influences myogenic differentiation.\n                  <\/jats:p>","DOI":"10.1096\/fj.202401285rr","type":"journal-article","created":{"date-parts":[[2024,11,13]],"date-time":"2024-11-13T07:00:40Z","timestamp":1731481240000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Identification of novel transcription factors regulated by\n                    <scp>H3K27<\/scp>\n                    acetylation in myogenic differentiation of porcine skeletal muscle satellite cells"],"prefix":"10.1096","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-1174-1742","authenticated-orcid":false,"given":"Peng","family":"Zhou","sequence":"first","affiliation":[{"name":"Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education and Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, College of Animal Science and Technology Huazhong Agricultural University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-4995-0628","authenticated-orcid":false,"given":"Wenxuan","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Informatics Huazhong Agricultural University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-4118-1333","authenticated-orcid":false,"given":"Jingjin","family":"Li","sequence":"additional","affiliation":[{"name":"Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education and Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, College of Animal Science and Technology Huazhong Agricultural University  Wuhan China"},{"name":"Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Key Laboratory of Livestock and Poultry Multi\u2010Omics of MARA, 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