{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T17:48:09Z","timestamp":1790876889343,"version":"4.1.0"},"reference-count":58,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2024,10,19]],"date-time":"2024-10-19T00:00:00Z","timestamp":1729296000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2024,10,19]],"date-time":"2024-10-19T00:00:00Z","timestamp":1729296000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2024,10,31]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Embryo survival and pre\u2010implantation development depend on uterine luminal fluid, which is believed to play a role in early embryonic death and infertility in cows. Extracellular vesicles (EVs) in the uterine luminal fluid contain microRNAs (miRNAs), crucial mediators of intercellular communication. miRNAs regulate conceptus\u2013maternal interactions and participate in embryonic development by suppressing gene expression. Therefore, we hypothesized that miRNAs in the intrauterine EVs of low\u2010fertility cows would hinder embryonic survival and development. EVs were collected from the bovine uterine luminal fluid of both normal\u2010 and low\u2010fertility cows 7\u2009days post\u2010estrus. Small RNA\u2010sequencing analysis of miRNAs isolated from these EVs identified eight miRNAs that were highly expressed in normal\u2010fertility cows (normal\u2010fertility miRNAs) and eight with elevated expression in low\u2010fertility cows (low\u2010fertility miRNAs). These two sets of miRNAs were transfected into hatched blastocysts via lipofection. RNA\u2010seq following lipofection with low\u2010fertility miRNAs identified 424 differentially expressed genes (DEGs) relative to the control; in contrast, following lipofection with normal\u2010fertility miRNAs, seven DEGs were identified. Pathway analysis of the DEGs identified following lipofection with low\u2010fertility miRNAs revealed substantial enrichment of mitogen\u2010activated protein kinase (MAPK) signaling. Expression of activator protein 1 (\n                    <jats:italic>AP1<\/jats:italic>\n                    ) and interferon\u2010tau\n                    <jats:italic>(IFNT)<\/jats:italic>\n                    mRNA was significantly lower in the low\u2010fertility miRNA transfection group than in the control. IFNT is essential for maternal pregnancy recognition. Therefore, miRNAs in intrauterine EVs from low\u2010fertility cows at 7\u2009days post\u2010estrus may inhibit embryo development and suppress\n                    <jats:italic>IFNT<\/jats:italic>\n                    expression by altering MAPK signaling.\n                  <\/jats:p>","DOI":"10.1096\/fj.202401728r","type":"journal-article","created":{"date-parts":[[2024,10,19]],"date-time":"2024-10-19T04:25:09Z","timestamp":1729311909000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Effects of intrauterine extracellular vesicle\n                    <scp>microRNAs<\/scp>\n                    on embryonic gene expression in low\u2010fertility cows"],"prefix":"10.1096","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-7849-600X","authenticated-orcid":false,"given":"Rei","family":"Ichikawa","sequence":"first","affiliation":[{"name":"Laboratory of Animal Production Science, Graduate School of 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