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The mechanisms underlying PA is complex and incompletely understood. In this study, we investigated the role of extracellular matrix (ECM) protein biglycan (BGN) in PA using an oxygen\u2010induced retinopathy (OIR) mouse model, along with hypoxia (1% O\n                    <jats:sub>2<\/jats:sub>\n                    ) conditions for incubating pericytes and endothelial cells in\u00a0vitro. We found a significant upregulation of\n                    <jats:italic>Bgn<\/jats:italic>\n                    in the retinas of OIR mice. Intravitreal injection of\n                    <jats:italic>Bgn<\/jats:italic>\n                    \u2010specific small interfering RNA (siRNA) in OIR mice at postnatal day 12 (P12) effectively curbed retinal PA at P17. Using cultured cells, we found that\n                    <jats:italic>BGN<\/jats:italic>\n                    expression in pericytes was highly sensitive to hypoxic stimulation compared to endothelial cells. We further showed that BGN stimulated retinal PA via the upregulation of C\u2010X\u2010C motif chemokine ligand 12 (CXCL12). Inhibition of the CXCL12\u2010CXCR4 axis effectively diminished PA in OIR mouse. In conclusion, our study demonstrated the stimulatory role of BGN in retinal PA, identified the link between BGN and CXCL12 expression, and further highlighted the role of pericytes in retinal PA.\n                  <\/jats:p>","DOI":"10.1096\/fj.202401903r","type":"journal-article","created":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T05:40:34Z","timestamp":1736142034000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Biglycan stimulates retinal pathological angiogenesis via up\u2010regulation of\n                    <scp>CXCL12<\/scp>\n                    expression in pericytes"],"prefix":"10.1096","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-9588-0692","authenticated-orcid":false,"given":"Miaomiao","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Ophthalmology Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine  Shanghai 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