{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T15:25:55Z","timestamp":1793460355834,"version":"4.5.0"},"reference-count":45,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,1,21]],"date-time":"2025-01-21T00:00:00Z","timestamp":1737417600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["RS\u20102023\u201000220894"],"award-info":[{"award-number":["RS\u20102023\u201000220894"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2025,1,31]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Sarcopenia is an age\u2010related muscle atrophy syndrome characterized by the loss of muscle strength and mass. Although many agents have been used to treat sarcopenia, there are no successful treatments to date. In this study, we identified Danshensu sodium salt (DSS) as a substantial suppressive agent of muscle atrophy. We used a D\u2010galactose (DG)\u2010induced aging\u2010acceleration model, both in\u00a0vivo and in\u00a0vitro, to confirm the effect of DSS on sarcopenia. DSS inhibits the expression of muscle atrophy\u2010related factors (MuRF1, MAFbx, myostatin, and FoxO3a) in DG\u2010induced mouse C2C12 and human skeletal muscle cells. Additionally, DSS restored the diameter of reduced C2C12 myotubes. Next, we demonstrated that DSS stimulates AMPK and PGC1\u03b1 through CaMKII. DSS inhibits the translocation of FoxO3a into the nucleus, thus inhibiting muscle atrophy in a calcium\u2010dependent manner. DSS initiated the protein\u2013protein interaction between FoxO3a and PGC1\u03b1. The reduction of the PGC1\u03b1\u2010FoxO3a interaction by DG was restored by DSS. Also, DSS suppressed increased intracellular reactive oxygen species (ROS) by DG. In animal models, DSS administration improved mouse muscle mass and physical performance (grip strength and hanging test) under DG\u2010induced accelerated aging conditions. These findings demonstrated that DSS attenuates muscle atrophy by inhibiting the expression of muscle atrophy\u2010related factors. Therefore, DSS may be a potential therapeutic agent for the treatment of sarcopenia.<\/jats:p>","DOI":"10.1096\/fj.202402158r","type":"journal-article","created":{"date-parts":[[2025,1,21]],"date-time":"2025-01-21T08:18:00Z","timestamp":1737447480000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Danshensu sodium salt alleviates muscle atrophy via\n                    <scp>CaMKII<\/scp>\n                    \u2010\n                    <scp>PGC1\u03b1<\/scp>\n                    \u2010\n                    <scp>FoxO3a<\/scp>\n                    signaling pathway in D\u2010galactose\u2010induced models"],"prefix":"10.1096","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-4209-4591","authenticated-orcid":false,"given":"Pooreum","family":"Lim","sequence":"first","affiliation":[{"name":"Department of Anatomy Korea University College of Medicine  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-4759-0564","authenticated-orcid":false,"given":"Sang Woo","family":"Woo","sequence":"additional","affiliation":[{"name":"Department of Anatomy Korea University College of Medicine  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-6369-4091","authenticated-orcid":false,"given":"Jihye","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Anatomy Korea University College of Medicine  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-6469-3065","authenticated-orcid":false,"given":"Young Lim","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Anatomy Korea University College of Medicine  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9861-6521","authenticated-orcid":false,"given":"Jae Ho","family":"Shim","sequence":"additional","affiliation":[{"name":"Department of Anatomy Korea University College of Medicine  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5869-4338","authenticated-orcid":false,"given":"Hyeon Soo","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Anatomy Korea University College of Medicine  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,1,21]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2014.12.001"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2019.172710"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2023.106754"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41401-021-00714-4"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2023.115153"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/0963689718771889"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules28041551"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2012.11.005"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2020.9550"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00223-014-9915-y"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2013.05.019"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-14-0057"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2021.10.001"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm.2017.95"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meatsci.2023.109287"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu15224714"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2020.583478"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.13459"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2024.116415"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2023.128623"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms231911963"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.2c05246"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/2990843"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10787-022-00973-5"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M807397200"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2011.01.005"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2019.03.194"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu15081801"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jot.2020.04.002"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2017.01.017"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12709"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201700442R"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0062338"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.2040-4603.2022.tb00211.x"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/ddr.21846"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.2174\/1871527321666220329144538"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12833"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-40435-2"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2021.11945"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2017.03.070"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0607795103"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1177\/0023677217738268"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI172890"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21155236"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22158310"}],"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.202402158R","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T16:34:18Z","timestamp":1761669258000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fj.202402158R"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,21]]},"references-count":45,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2025,1,31]]}},"alternative-id":["10.1096\/fj.202402158R"],"URL":"https:\/\/doi.org\/10.1096\/fj.202402158r","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"value":"0892-6638","type":"print"},{"value":"1530-6860","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,21]]},"assertion":[{"value":"2024-09-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-21","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70280"}}