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This study aimed to identify novel circulating inflammatory biomarkers for improved ICH prediction and diagnosis. We profiled expression levels of 92\u00a0cardiovascular disease related proteins in plasma from 26 matched ICH patients and controls using Olink technology. Differentially expressed proteins were validated using ELISA and RT\u2010qPCR in a second matched cohort. Receiver operating characteristic (ROC) curves evaluated how well the diagnostic tests performed. The study identified 18 inflammatory\u2010related proteins with significantly different expression levels between ICH patients and controls. These proteins participate in critical biological processes and pathways, such as the regulation of inflammatory mediator secretion, cell death, immune cell proliferation and differentiation, pathogen response, and PI3K\u2010Akt and JAK\u2013STAT pathways. Notably, we discovered for the first time that Kidney Injury Molecule\u20101 (KIM1) is significantly upregulated in the plasma of ICH patients, suggesting its potential as a predictive and diagnostic biomarker for ICH. Validation results from ELISA and RT\u2010qPCR showed that Interleukin\u20106 (IL\u20106), Pentraxin 3 (PTX3), KIM1, and Galectin\u20109 (Gal\u20109) concentrations were markedly increased in the blood plasma and white matter of individuals with ICH. ROC analysis showed that the combined marker of IL\u20106, PTX3, KIM1 and Gal\u20109 had a high diagnostic efficacy (AUC\u2009=\u20090.941). This study identified a novel biomarker panel (IL\u20106, PTX3, KIM1, Gal\u20109) for ICH diagnosis. KIM1 upregulation in ICH patients is a novel finding, further investigation is needed into its expression and function in ICH.<\/jats:p>","DOI":"10.1096\/fj.202402183rr","type":"journal-article","created":{"date-parts":[[2025,1,24]],"date-time":"2025-01-24T10:29:44Z","timestamp":1737714584000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Novel inflammatory markers in intracerebral hemorrhage: Results from Olink proteomics analysis"],"prefix":"10.1096","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-4050-4015","authenticated-orcid":false,"given":"Ziliang","family":"Hu","sequence":"first","affiliation":[{"name":"Cixi Biomedical Research Institute Wenzhou Medical University  Ningbo Zhejiang China"},{"name":"Department of Neurosurgery, Ningbo Key Laboratory of Nervous System and Brain Function The First Affiliated Hospital of Ningbo University  Ningbo Zhejiang China"},{"name":"Key Laboratory of 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