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Successful gestation processes are closely related to a suitable endometrial microenvironment, and the physiological mechanisms leading to failed pregnancy during the peri\u2010implantation period remain unclear. We constructed single\u2010cell transcriptome profiles of peri\u2010implantation embryo loss and successful gestation endometrial tissues and identified 22 cell subpopulations, with epithelial and stromal cells being the predominant endometrial cell types. The two tissues showed marked differences in cell type composition, especially among epithelial cell subpopulations. We also observed functional differences between epithelial and stromal cells in tissues from embryonic loss and successful gestation, as well as the expression levels and differentiation trajectories of genes associated with embryo attachment and endometrial receptivity in epithelial and stromal cells. 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