{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T16:42:31Z","timestamp":1761669751794,"version":"build-2065373602"},"reference-count":53,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,1,24]],"date-time":"2025-01-24T00:00:00Z","timestamp":1737676800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2025,1,31]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Adipose tissue (AT), is a major endocrine organ that plays a key role in health and disease. However, adipose dysfunctions, especially altered energy metabolism, have been under\u2010investigated as white adipocytes have relatively low mitochondrial density. Nevertheless, recent studies suggest that mitochondria could play a major role in AT disorders and that AT mitochondrial activity could depend on adiposity level and location. This clinical study aimed to evaluate mitochondrial respiration and metabolism in human visceral (vAT) and subcutaneous (scAT) AT and their relationship with body mass index (BMI). This clinical study enrolled 67 patients (30 females\/37 males) scheduled for digestive surgery without chemotherapy and parietal infection. BMI ranged from 15.4 to 51.9\u2009kg\u00b7m\n                    <jats:sup>\u22122<\/jats:sup>\n                    and body composition was estimated by computed tomographic images. Mitochondrial respiration was measured in\u00a0situ in digitonin\u2010permeabilized AT using high\u2010resolution respirometry and a substrate\/inhibitor titration approach. Protein levels of mitochondrial and lipid metabolism key elements were evaluated by Western blot. Maximal mitochondrial respiration correlated negatively with BMI (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u2009.01) and AT area (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u2009.001) regardless of the anatomical location. However, oxidative phosphorylation respiration was significantly higher in vAT (2.22\u2009\u00b1\u20090.15\u2009pmol\u00b7sec\n                    <jats:sup>\u22121<\/jats:sup>\n                    \u00b7mg\n                    <jats:sup>\u22121<\/jats:sup>\n                    ) than scAT (1.79\u2009\u00b1\u20090.17\u2009pmol\u00b7sec\n                    <jats:sup>\u22121<\/jats:sup>\n                    \u00b7mg\n                    <jats:sup>\u22121<\/jats:sup>\n                    ) (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001). In line with oxygraphy results, there were higher levels of mitochondrial respiratory chain complexes in low\u2010BMI patients and vAT. Mitochondrial respiration decreased with increasing BMI in both scAT and vAT, without sex\u2010associated difference. Mitochondrial respiration appeared to be higher in vAT than scAT. These differences were both qualitative and quantitative. Clinical Trials Registration\n                    <jats:styled-content style=\"fixed-case\">ID<\/jats:styled-content>\n                    NCT05417581.\n                  <\/jats:p>","DOI":"10.1096\/fj.202402243r","type":"journal-article","created":{"date-parts":[[2025,1,25]],"date-time":"2025-01-25T00:00:42Z","timestamp":1737763242000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Mitochondrial respiration in white adipose tissue is dependent on body mass index and tissue location in patients undergoing oncological or parietal digestive surgery"],"prefix":"10.1096","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-3013-6154","authenticated-orcid":false,"given":"Lisa","family":"Guerrier","sequence":"first","affiliation":[{"name":"INRAe, Human Nutrition Unit Clermont Auvergne University  Clermont\u2010Ferrand 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Radiology CHU Gabriel Montpied  Clermont\u2010Ferrand France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucie","family":"Cassagnes","sequence":"additional","affiliation":[{"name":"Department of Radiology CHU Gabriel Montpied  Clermont\u2010Ferrand France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4382-8735","authenticated-orcid":false,"given":"Aur\u00e9lie","family":"Vieille\u2010Marchiset","sequence":"additional","affiliation":[{"name":"INRAe, INSERM, CarMeN Laboratory Claude Bernard Lyon 1 University  Lyon France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6930-6671","authenticated-orcid":false,"given":"St\u00e9phanie","family":"Chanon","sequence":"additional","affiliation":[{"name":"INRAe, INSERM, CarMeN Laboratory Claude Bernard Lyon 1 University  Lyon 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