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Much effort has been made to develop varied types of NOX inhibitors, but biotechnologies for spatially and temporally controlled NOX activation, however, are not readily available. We previously found that ultraviolet A (UVA) irradiation activates NOX2 in rodent mast cells, to elicit persistent calcium spikes. NOX2 is composed of multiple subunits, making studies of its activation rather complicated. Here we show that the single\u2010subunit nonrodent\u2010expressing NOX5, when expressed ectopically in CHO\u2010K1 cells, is activated by UVA irradiation (380\u2009nm, 0.1\u201312\u2009mW\/cm\n                    <jats:sup>2<\/jats:sup>\n                    , 1.5\u2009min) inducing repetitive calcium spikes, as monitored by Fura\u20102 fluorescent calcium imaging. UVA\u2010elicited calcium oscillations are inhibited by NOX inhibitor diphenyleneiodonium chloride (DPI) and blocked by singlet oxygen (\n                    <jats:sup>1<\/jats:sup>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    ) quencher Trolox\u2010C (300\u2009\u03bcM). A brief pulse of photodynamic action (1.5 min) with photosensitizer sulfonated aluminum phthalocyanine (SALPC 2\u2009\u03bcM, 675\u2009nm, 85\u2009mW\/cm\n                    <jats:sup>2<\/jats:sup>\n                    ) in NOX5\u2010CHO\u2010K1 cells, or with genetically encoded protein photosensitizer miniSOG fused to N\u2010terminus of NOX5 (450\u2009nm, 85\u2009mW\/cm\n                    <jats:sup>2<\/jats:sup>\n                    ) in miniSOG\u2010NOX5\u2010CHO\u2010K1 cells, induces persistent calcium oscillations, which are blocked by DPI. In the presence of Trolox\u2010C, miniSOG photodynamic action no longer induces any calcium increases in miniSOG\u2010NOX5\u2010CHO\u2010K1 cells. DUOX2 in human thyroid follicular cells SW579 and in DUOX2\u2010CHO\u2010K1 cells is similarly activated by UVA irradiation and SALPC photodynamic action. These data together suggest that NOX \u00a0 is activated with a brief pulse of photodynamic action.\n                  <\/jats:p>","DOI":"10.1096\/fj.202402292r","type":"journal-article","created":{"date-parts":[[2024,12,10]],"date-time":"2024-12-10T06:55:18Z","timestamp":1733813718000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["To activate\n                    <scp>NAD<\/scp>\n                    (P)H oxidase with a brief pulse of photodynamic action"],"prefix":"10.1096","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-1921-6614","authenticated-orcid":false,"given":"Xiao Bing","family":"Xie","sequence":"first","affiliation":[{"name":"College of Life Sciences Beijing Normal University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Shu","sequence":"additional","affiliation":[{"name":"College of Life Sciences Beijing Normal University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4252-4876","authenticated-orcid":false,"given":"Zong Jie","family":"Cui","sequence":"additional","affiliation":[{"name":"College of Life Sciences Beijing Normal University  Beijing 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