{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,2]],"date-time":"2026-08-02T23:46:45Z","timestamp":1785714405676,"version":"3.56.0"},"reference-count":54,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,5,6]],"date-time":"2025-05-06T00:00:00Z","timestamp":1746489600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000265","name":"Medical Research Council","doi-asserted-by":"publisher","award":["MR\/M02184X\/1"],"award-info":[{"award-number":["MR\/M02184X\/1"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2025,5,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Maternal uterine spiral arteries (SpA) undergo significant structural changes in early pregnancy, resulting in increased blood flow to the developing fetus. Endothelial cells (EC) and vascular smooth muscle cells (VSMC) are lost from the SpA wall and are replaced by trophoblasts. We have previously shown that matrix metalloproteinase 10 (MMP\u201010) and Heparin binding\u2010EGF like growth factor (HB\u2010EGF) gene expression is increased in a 3D EC\/VSMC co\u2010culture system in response to trophoblast secreted factors. This study investigated trophoblast mediated MMP\u201010 and HB\u2010EGF expression and determined if there was a relationship between the secretion of MMP\u201010 and the release of soluble HB\u2010EGF (sHB\u2010EGF) from EC. MMP\u201010 was widely expressed in first trimester decidual tissue including trophoblast, and EC, but not VSMC. MMP\u201010 expression was significantly lower in decidual tissue from pregnancies at increased risk of developing pre\u2010eclampsia compared to low\u2010risk pregnancies. In\u00a0vitro, SGHEC\u20107 cells, a human EC line, but not SGHVMC\u20109, a human VSMC cell line, secreted MMP\u201010 in response to trophoblast conditioned medium (TCM). TCM contains several growth factors and cytokines, but only interleukin\u20101\u03b2 (IL1\u03b2) significantly stimulated MMP\u201010 secretion by SGHEC\u20107 cells. Interleukin\u20101 receptor antagonist (IL\u20101Ra) significantly inhibited TCM\u2010induced MMP\u201010 secretion. Interrogation of intracellular pathways established the involvement of MEK and JNK in TCM and IL\u20101\u03b2 stimulated MMP\u201010 secretion. Although IL\u20101\u03b2 also significantly increased sHB\u2010EGF, inhibition of MMP\u201010 activity using a broad spectrum MMP inhibitor had no effect on sHB\u2010EGF. Western blot analysis indicated that MMP\u201010 secreted by EC in response to IL\u20101\u03b2 stimulation was the enzymatically inactive pro form.<\/jats:p>","DOI":"10.1096\/fj.202402329rr","type":"journal-article","created":{"date-parts":[[2025,5,6]],"date-time":"2025-05-06T09:04:14Z","timestamp":1746522254000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Interleukin\u20101\u03b2 Stimulates Matrix Metalloproteinase 10 Secretion: A Possible Mechanism in Trophoblast\u2010Dependent Spiral Artery Remodeling"],"prefix":"10.1096","volume":"39","author":[{"given":"Holly","family":"Tinsley","sequence":"first","affiliation":[{"name":"Centre for Vascular Biology, Cardiovascular and Genomics Research Institute St George's, University of London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zoe","family":"Tryfonos","sequence":"additional","affiliation":[{"name":"Centre for Vascular Biology, Cardiovascular and Genomics Research Institute St George's, University of London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miriam","family":"Aziz","sequence":"additional","affiliation":[{"name":"Centre for Vascular Biology, Cardiovascular and Genomics Research Institute St George's, University of London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nora","family":"Lagzouli","sequence":"additional","affiliation":[{"name":"Centre for Vascular Biology, Cardiovascular and Genomics Research Institute St George's, University of London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlotte","family":"Longhurst","sequence":"additional","affiliation":[{"name":"Centre for Vascular Biology, Cardiovascular and Genomics Research Institute St George's, University of London  London UK"},{"name":"Royal Devon University Trust  Exeter UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Frick","sequence":"additional","affiliation":[{"name":"Fetal Medicine Unit St George's University Hospitals NHS Foundation Trust  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Ashton","sequence":"additional","affiliation":[{"name":"Centre for Vascular Biology, Cardiovascular and Genomics Research Institute St George's, University of London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judith E.","family":"Cartwright","sequence":"additional","affiliation":[{"name":"Centre for Vascular Biology, Cardiovascular and Genomics Research Institute St George's, University of London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9670-3004","authenticated-orcid":false,"given":"Guy S.","family":"Whitley","sequence":"additional","affiliation":[{"name":"Centre for Vascular Biology, Cardiovascular and Genomics Research Institute St George's, University of London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,5,6]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2009.02.009"},{"key":"e_1_2_11_3_1","first-page":"557","article-title":"Changes in Vascular Extracellular Matrix Composition During Decidual Spiral Arteriole Remodeling in Early Human Pregnancy","volume":"31","author":"Smith S. 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