{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T11:08:42Z","timestamp":1793272122530,"version":"4.5.0"},"reference-count":35,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,2,28]],"date-time":"2025-02-28T00:00:00Z","timestamp":1740700800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100018542","name":"Natural Science Foundation of Sichuan Province","doi-asserted-by":"publisher","award":["2022NSFSC1373"],"award-info":[{"award-number":["2022NSFSC1373"]}],"id":[{"id":"10.13039\/501100018542","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2025,3,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Preeclampsia (PE) is one of the most dangerous complications of pregnancy. The pathogenic mechanisms of this condition are not yet clear. Lysine lactylation (Kla) is a novel post\u2010translational modification (PTM) reported recently. It remains to be determined whether Kla plays a role in the development of PE. Here, western blotting revealed that the placental Kla profile of PE was different from that of normal pregnancies, and hydrogen peroxide (H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    ) weakened the Kla level of trophoblast cells. High\u2010performance liquid chromatography\u2013tandem mass spectrometry (HPLC\u2013MS\/MS) indicated that 333 Kla sites of 232 proteins were changed by Kla in BeWo cells (a trophoblast cell line) treated with H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    , among which only HK2 showed a unique Kla site (K346) with down\u2010regulated lactylation. Additionally, the inactive mutant HK2\u2010K346 was associated with decreased hexokinase activity, lower affinity to voltage\u2010dependent anion channel 1 (VDAC1), and impaired cell proliferation. These findings demonstrate that lactylation is involved in the pathogenesis of PE and that lactylation of HK2\u2010K346 could serve as a new connection between oxidative stress, energy metabolism, and the development of PE.\n                  <\/jats:p>","DOI":"10.1096\/fj.202402430rr","type":"journal-article","created":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T12:01:44Z","timestamp":1741694504000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Oxidative stress regulates the catalytic activity and mitochondrial localization of\n                    <scp>HK2<\/scp>\n                    in trophoblast by regulating\n                    <scp>K346<\/scp>\n                    lactylation"],"prefix":"10.1096","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7124-8941","authenticated-orcid":false,"given":"Zhirui","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Obstetrics 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Soochow University  Suzhou Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2323-2926","authenticated-orcid":false,"given":"Yun","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Maternal and Child Health Hospital of Hubei Province, Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5589-3836","authenticated-orcid":false,"given":"Haixia","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Union Hospital, Tongji Medical College, Huazhong University of Science and Technology  Wuhan China"},{"name":"Department of Reproductive Medicine The First Affiliated Hospital of Nanchang University  Nanchang Jiangxi 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