{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T18:44:07Z","timestamp":1770749047246,"version":"3.50.0"},"reference-count":59,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2025,7,18]],"date-time":"2025-07-18T00:00:00Z","timestamp":1752796800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100007225","name":"Ministry of Science and Technology","doi-asserted-by":"publisher","award":["110\u20102314\u2010B\u2010006\u2010088"],"award-info":[{"award-number":["110\u20102314\u2010B\u2010006\u2010088"]}],"id":[{"id":"10.13039\/100007225","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2025,7,31]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Proliferative vitreoretinopathy (PVR) is a severe complication after rhegmatogenous retinal detachment (RD) surgery and needs further surgical treatments. So far there are no effective drugs for prevention and treatment of PVR. Both integrins \u03b1v\u03b23 and \u03b15\u03b21 are related to human PVR formation. Disintegrins are antagonists of integrins. The drug, a mutant of snake venom\u2010derived disintegrin, also called disintegrin Rhodostomin (Rho) mutant, is a potent antagonist of both integrins \u03b1v\u03b23 and \u03b15\u03b21, of which the effect on PVR remains poorly understood. In\u00a0vitro assays were used to assess the effects of disintegrin Rho mutant treatment on the proliferation, migration, and adhesion of the retinal pigment epithelial (RPE) cell line derived from human, ARPE\u201019. In\u00a0vivo, mice with PVR induction were treated with disintegrin Rho mutant and monitored for PVR severity. In\u00a0vitro results showed that disintegrin Rho mutant reduced the migration, proliferation, and adhesion of ARPE\u201019 cells. In\u00a0vivo results revealed that it could significantly ameliorate the severity of PVR and suppress expression of both integrin \u03b1v\u03b23 and \u03b15\u03b21 and epithelial\u2010mesenchymal transition (EMT) in mouse eyes. Besides, we found decreases in Akt, STAT3, and ERK activation by disintegrin Rho mutant both in\u00a0vitro and in\u00a0vivo. In conclusion, disintegrin Rho mutant mitigates experimental PVR with reduced expression of both integrin \u03b1v\u03b23 and \u03b15\u03b21 and presents a potential therapeutic option for PVR in humans.<\/jats:p>","DOI":"10.1096\/fj.202402498rr","type":"journal-article","created":{"date-parts":[[2025,7,18]],"date-time":"2025-07-18T09:00:53Z","timestamp":1752829253000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Disintegrin Rhodostomin Mutant Ameliorates the Severity of Experimental Proliferative Vitreoretinopathy by Suppressing Both Integrin \u03b1v\u03b23 and \u03b15\u03b21"],"prefix":"10.1096","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6692-7608","authenticated-orcid":false,"given":"Yu\u2010Chien","family":"Tsao","sequence":"first","affiliation":[{"name":"Department of Ophthalmology National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University  Tainan Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shun\u2010Hua","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology College of Medicine, National Cheng Kung University  Tainan Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi\u2010Chun","family":"Ou","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology College of Medicine, National Cheng Kung University  Tainan Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Woei\u2010Jer","family":"Chuang","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology College of Medicine, National Cheng Kung University  Tainan Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4328-8716","authenticated-orcid":false,"given":"Chang\u2010Hao","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology National Taiwan University Hospital, College of Medicine, National Taiwan University  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chia\u2010Jhen","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University  Tainan Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4029-4125","authenticated-orcid":false,"given":"Sheng\u2010Min","family":"Hsu","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University  Tainan Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,7,18]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/IIO.0000000000000258"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.1981.03930010873017"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1350-9462(97)00007-4"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2020.00501"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2015.02.008"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmjophth-2019-000293"},{"key":"e_1_2_10_8_1","first-page":"88","article-title":"The Pathogeny of Proliferative Vitreoretinopathy","volume":"59","author":"Ciprian D.","year":"2015","journal-title":"Romanian Journal of Ophthalmology"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41390-020-01177-9"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2020.588066"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0510774103"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2016.02.040"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2021.100966"},{"key":"e_1_2_10_14_1","first-page":"3475","article-title":"Immunolocalization of Integrins in Proliferative Retinal Membranes","volume":"35","author":"Robbins S. 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