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Although the mechanisms governing bradyzoite differentiation have been partially elucidated, they remain incompletely understood. In this study, we show that the transcription factor AP2XII\u20109 is localized in the nucleus and exhibits periodic expression during the tachyzoite stage, with peak expression observed during the synthesis and mitosis phases. Conditional knockdown of AP2XII\u20109 in both the type I RH strain and type II cyst\u2010forming Pru strain revealed that AP2XII\u20109 plays a critical role in the lytic cycle by regulating the formation of the inner membrane complex, proper apicoplast inheritance, and normal cell division, underscoring its essential role in\n                    <jats:italic>T. gondii<\/jats:italic>\n                    growth. Furthermore, depletion of AP2XII\u20109 induced bradyzoite differentiation even in the absence of alkaline stress. Transcriptomic analysis revealed that the deletion of AP2XII\u20109 resulted in the downregulation of tachyzoite growth\u2010related genes and upregulation of a series of bradyzoite\u2010specific genes. Taken together, these findings indicate that AP2XII\u20109 is essential for maintaining the rapid and normal replication of tachyzoites while actively repressing bradyzoite differentiation, reflecting the complexity of the mechanisms underlying bradyzoite differentiation.\n                  <\/jats:p>","DOI":"10.1096\/fj.202402593rr","type":"journal-article","created":{"date-parts":[[2025,3,19]],"date-time":"2025-03-19T12:31:54Z","timestamp":1742387514000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["AP2XII\u20109 is essential for parasite growth and suppresses bradyzoite differentiation in\n                    <i>Toxoplasma gondii<\/i>"],"prefix":"10.1096","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-3724-0776","authenticated-orcid":false,"given":"Xiao\u2010Jing","family":"Wu","sequence":"first","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention, Key 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