{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T14:06:50Z","timestamp":1771510010313,"version":"3.50.1"},"reference-count":26,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,5,8]],"date-time":"2025-05-08T00:00:00Z","timestamp":1746662400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82172235"],"award-info":[{"award-number":["82172235"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2025,5,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Androgenetic alopecia (AGA) is a prevalent form of hair loss with poorly understood mechanisms. This study investigates the association between the tumor necrosis factor receptor superfamily member 1B (TNFRSF1B) gene and AGA, aiming to validate its potential causal relationship. Transcriptomic data from AGA patients were obtained from the Gene Expression Omnibus (GEO) database to identify differentially expressed genes (DEGs). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses, along with Weighted Gene Co\u2010expression Network Analysis (WGCNA), were employed to determine AGA\u2010related key genes. We then performed Mendelian randomization (MR) analysis with genome\u2010wide association study (GWAS) data to assess the causal relationship between key genes and AGA, employing methods such as weighted median and MR\u2010Egger regression for sensitivity analysis. Meta\u2010analysis of AGA\u2010related datasets from GEO was conducted, followed by in\u00a0vitro and in\u00a0vivo experiments on TNFRSF1B to evaluate its impact on hair dermal papilla cells (HDPCs) and AGA models. RNA\u2010seq data revealed significant upregulation of TNFRSF1B, PIK3CD, and THEMIS2 in AGA patient hair follicles. ROC analysis indicated their diagnostic potential, with WGCNA and gene intersection analysis identifying TNFRSF1B as closely associated with AGA. MR confirmed a causal relationship between TNFRSF1B and AGA. Meta\u2010analysis further validated TNFRSF1B upregulation across multiple AGA datasets. In\u00a0vitro experiments demonstrated that TNFRSF1B knockdown enhanced HDPC proliferation and migration, reduced apoptosis, and upregulated \u03b2\u2010catenin and CyclinD1 expression. Additionally, TNFRSF1B knockdown reduced ROS levels and increased Catalase and SOD2 expression under oxidative stress. In\u00a0vivo, TNFRSF1B knockdown promoted hair regeneration and reduced oxidative stress in an AGA mice model. The findings suggest that TNFRSF1B is a potential pathogenic factor in AGA, providing a novel therapeutic target for intervention.<\/jats:p>","DOI":"10.1096\/fj.202402832r","type":"journal-article","created":{"date-parts":[[2025,5,12]],"date-time":"2025-05-12T09:57:23Z","timestamp":1747043843000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The\n                    <scp>TNFRSF1B<\/scp>\n                    Connection: Implications for Androgenetic Alopecia Pathogenesis and Treatment"],"prefix":"10.1096","volume":"39","author":[{"given":"Feihong","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Plastic and Aesthetic Surgery Nanfang Hospital of Southern Medical University  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ye","family":"He","sequence":"additional","affiliation":[{"name":"Department of Plastic and Aesthetic Surgery Nanfang Hospital of Southern 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