{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T15:16:54Z","timestamp":1777648614002,"version":"3.51.4"},"reference-count":10,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T00:00:00Z","timestamp":1736121600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000069","name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","doi-asserted-by":"publisher","award":["R01AR077145"],"award-info":[{"award-number":["R01AR077145"]}],"id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["R01HL114682"],"award-info":[{"award-number":["R01HL114682"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2025,1,15]]},"abstract":"Abstract<\/jats:title>\n \n Aging is a risk factor for several chronic conditions, including intervertebral disc degeneration and associated back pain. Disc pathologies include loss of reticular\u2010shaped nucleus pulposus cells, disorganization of annulus fibrosus lamellae, reduced disc height, and increased disc bulging. Sonic hedgehog, cytokeratin 19, and extracellular matrix proteins are markers of healthy disc. Preclinical murine models help understand cellular and molecular mechanisms of disc pathologies and the associated neurological symptoms. Klotho (\n Kl<\/jats:italic>\n ) is a known anti\u2010aging gene.\n Kl<\/jats:italic>\n \u2010deficient mice (\n Kl<\/jats:italic>\n \n Kl<\/jats:italic>\n <\/jats:sup>\n \/Kl<\/jats:italic>\n \n Kl<\/jats:italic>\n <\/jats:sup>\n ) have a reduced lifespan and display accelerated aging phenotypes in several tissues, including the kidney, pancreas, bone, and brain. To test the suitability of\n Kl<\/jats:italic>\n \n Kl<\/jats:italic>\n <\/jats:sup>\n \/Kl<\/jats:italic>\n \n Kl<\/jats:italic>\n <\/jats:sup>\n mice for studying accelerated disc pathologies, we characterized the lumbar discs of eight\u2010week\u2010old\n Kl<\/jats:italic>\n \n Kl<\/jats:italic>\n <\/jats:sup>\n \/Kl<\/jats:italic>\n \n Kl<\/jats:italic>\n <\/jats:sup>\n mice compared to wild\u2010type controls. No structural, morphological, or molecular differences were observed in the discs of\n Kl<\/jats:italic>\n \n Kl<\/jats:italic>\n <\/jats:sup>\n \/Kl<\/jats:italic>\n \n Kl<\/jats:italic>\n <\/jats:sup>\n mice compared to controls. Next, we tested the hypothesis that\n Kl<\/jats:italic>\n \n Kl<\/jats:italic>\n <\/jats:sup>\n \/Kl<\/jats:italic>\n \n Kl<\/jats:italic>\n <\/jats:sup>\n mice do not display accelerated disc pathologies due to the absence of\n Kl<\/jats:italic>\n expression or response by disc cells. Multiplex qPCR analysis did not detect any\n Kl<\/jats:italic>\n isoforms in the disc cells, explaining the absence of disc phenotype in\n Kl<\/jats:italic>\n \n Kl<\/jats:italic>\n <\/jats:sup>\n \/Kl<\/jats:italic>\n \n Kl<\/jats:italic>\n <\/jats:sup>\n mutants.\n <\/jats:p>","DOI":"10.1096\/fj.202402847r","type":"journal-article","created":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T05:30:32Z","timestamp":1736141432000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Klotho mutation does not accelerate intervertebral disc aging in mice"],"prefix":"10.1096","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-4025-5980","authenticated-orcid":false,"given":"Justin","family":"Hong","sequence":"first","affiliation":[{"name":"HSS Research Institute Hospital for Special Surgery New York New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-1935-0566","authenticated-orcid":false,"given":"Veeraj","family":"Shah","sequence":"additional","affiliation":[{"name":"HSS Research Institute Hospital for Special Surgery New York New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-4211-9732","authenticated-orcid":false,"given":"Ravi","family":"Krishnan","sequence":"additional","affiliation":[{"name":"HSS Research Institute Hospital for Special Surgery New York New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. Victoria","family":"Gomez\u2010Stallons","sequence":"additional","affiliation":[{"name":"Division of Molecular Cardiovascular Biology, The Heart Institute Cincinnati Children's Hospital Medical Center Cincinnati Ohio USA"},{"name":"Department of Pediatrics University of Cincinnati College of Medicine Cincinnati Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sunil","family":"Poudel","sequence":"additional","affiliation":[{"name":"HSS Research Institute Hospital for Special Surgery New York New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katherine E.","family":"Yutzey","sequence":"additional","affiliation":[{"name":"Division of Molecular Cardiovascular Biology, The Heart Institute Cincinnati Children's Hospital Medical Center Cincinnati Ohio USA"},{"name":"Department of Pediatrics University of Cincinnati College of Medicine Cincinnati Ohio USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3683-9791","authenticated-orcid":false,"given":"Chitra L.","family":"Dahia","sequence":"additional","affiliation":[{"name":"HSS Research Institute Hospital for Special Surgery New York New York USA"},{"name":"Department of Cell and Developmental Biology Weill Cornell Medical College New York New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,1,6]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/acel.13006"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/36285"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10157-012-0621-7"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0056695"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2009121311"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1640239"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2007.02.002"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/8319516"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mad.2022.111715"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S8756-3282(02)00786-X"}],"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.202402847R","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T18:37:13Z","timestamp":1761676633000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fj.202402847R"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,6]]},"references-count":10,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1,15]]}},"alternative-id":["10.1096\/fj.202402847R"],"URL":"https:\/\/doi.org\/10.1096\/fj.202402847r","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"value":"0892-6638","type":"print"},{"value":"1530-6860","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,6]]},"assertion":[{"value":"2024-11-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70279"}}