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However, the expression of FOS and function of FOS have not been determined in the mouse ovary. FOS, a member of the Fos family (\n                    <jats:italic>Fos, Fosb, Fosl1<\/jats:italic>\n                    , and\n                    <jats:italic>Fosl2<\/jats:italic>\n                    ), functions as a transcription factor by forming a heterodimer complex with a member of Jun family (\n                    <jats:italic>Jun, Junb<\/jats:italic>\n                    , and\n                    <jats:italic>Jund<\/jats:italic>\n                    ). This study demonstrated rapid increases in\n                    <jats:italic>Fos<\/jats:italic>\n                    , along with other Fos and Jun family members, after hCG administration in the ovary of immature PMSG\u2010primed mice and after the LH surge in naturally cycling animals. ChIP\u2010seq analysis identified 1965 FOS\u2010binding genes in granulosa cells collected at 3\u2009h post\u2010hCG, including\n                    <jats:italic>Pgr<\/jats:italic>\n                    ,\n                    <jats:italic>Ptgs2, Tnfiap6<\/jats:italic>\n                    , and\n                    <jats:italic>Edn2<\/jats:italic>\n                    , genes known to be involved in the ovulatory process. When super\u2010ovulation was induced, the number of oocytes released was significantly reduced in\n                    <jats:italic>Esr2<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>cre\/+<\/jats:italic>\n                    <\/jats:sup>\n                    \u2010driven granulosa cell\u2010specific\n                    <jats:italic>Fos<\/jats:italic>\n                    knockout (\n                    <jats:italic>gcFos<\/jats:italic>\n                    KO) mice. This reduction was accompanied by lower expressions of\n                    <jats:italic>Pgr, Ptgs2, Ptgs1,<\/jats:italic>\n                    and\n                    <jats:italic>Edn2<\/jats:italic>\n                    in preovulatory follicles of\n                    <jats:italic>gcFosKO<\/jats:italic>\n                    mice compared to those in control littermates. In addition,\n                    <jats:italic>gcFos<\/jats:italic>\n                    KO mice showed a trend toward a decreased average litter size. Together, the present study indicates that the preovulatory induction of\n                    <jats:italic>Fos<\/jats:italic>\n                    expression is crucial for increasing the expression of key ovulatory genes, yet the role of FOS may be partially substituted by other\n                    <jats:italic>Fos<\/jats:italic>\n                    and\n                    <jats:italic>Jun<\/jats:italic>\n                    family members induced in the preovulatory follicle in the\n                    <jats:italic>gcFos<\/jats:italic>\n                    KO mouse ovary.\n                  <\/jats:p>","DOI":"10.1096\/fj.202402867r","type":"journal-article","created":{"date-parts":[[2025,2,13]],"date-time":"2025-02-13T06:40:51Z","timestamp":1739428851000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Granulosa cell expression of\n                    <i>Fos<\/i>\n                    is critical for regulating 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