{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T18:41:19Z","timestamp":1783363279138,"version":"3.54.6"},"reference-count":43,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,4,2]],"date-time":"2025-04-02T00:00:00Z","timestamp":1743552000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81860451"],"award-info":[{"award-number":["81860451"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005273","name":"Natural Science Foundation of Yunnan Province","doi-asserted-by":"publisher","award":["202401AS070019"],"award-info":[{"award-number":["202401AS070019"]}],"id":[{"id":"10.13039\/501100005273","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005273","name":"Natural Science Foundation of Yunnan Province","doi-asserted-by":"publisher","award":["202201AY070001\u2010045"],"award-info":[{"award-number":["202201AY070001\u2010045"]}],"id":[{"id":"10.13039\/501100005273","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005273","name":"Natural Science Foundation of Yunnan Province","doi-asserted-by":"publisher","award":["202101AY070001\u2010014"],"award-info":[{"award-number":["202101AY070001\u2010014"]}],"id":[{"id":"10.13039\/501100005273","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005273","name":"Natural Science Foundation of Yunnan Province","doi-asserted-by":"publisher","award":["202001BA070001\u2010070"],"award-info":[{"award-number":["202001BA070001\u2010070"]}],"id":[{"id":"10.13039\/501100005273","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2025,4,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    STEAP4 manifested differential expression and aberrant methylation in prostate cancer (PCa). Therefore, this study proposed to explore the effect of STEAP4 on the PCa malignant phenotype in\u00a0vivo and in\u00a0vitro and the possible molecular mechanisms using RNA\u2010seq. The expression of STEAP4 in PCa and its prognostic and diagnostic value was identified using bioinformatics. After exogenous modulation of STEAP4, the effect of STEAP4 on the malignant phenotype of PCa cells was examined using functional assays and nude mouse tumor models. The STEAP4\u2010related differentially expressed genes (DEGs) and the hub genes were characterized using RNA\u2010seq in conjunction with bioinformatics. STEAP4 exhibited high expression in PCa tissues from TCGA\u2010PRAD and GEO datasets (GSE179321, GSE229904, and GSE237995), which predicted lower survival of patients. The STEAP4\u2010associated nomogram model and diagnostic ROC curve had excellent predictive performance (AUC\u2009=\u20090.814). STEAP4 was overexpressed in PCa tissues and cells. Knockdown of STEAP4 effectively decreased the viability, number of invading cells, and wound healing of PCa cells and increased apoptosis. Overexpression of STEAP4 showed the opposite pattern. RNA\u2010seq revealed that knockdown of STEAP4 resulted in 234 DEGs in PCa cells.\n                    <jats:italic>FGF17<\/jats:italic>\n                    ,\n                    <jats:italic>KCNQ2<\/jats:italic>\n                    ,\n                    <jats:italic>PDGFRB<\/jats:italic>\n                    , and\n                    <jats:italic>NOTCH4<\/jats:italic>\n                    are hub genes in DEGs. Notably, NOTCH4 was likewise overexpressed in PCa tissues and cells and was regulated by STEAP4. In in\u00a0vitro experiments, overexpression of NOTCH4 facilitated PCa cell proliferation, migration, and invasion, which was limited by knockdown of STEAP4. In in\u00a0vivo experiments, overexpression of STEAP4 exacerbated PCa tumor burden, which was rescued by knockdown of NOTCH4. STEAP4 is a valid biomarker for predicting prognosis and diagnosis of PCa patients. STEAP4 contributes to PCa growth, migration, and invasion by upregulating NOTCH4.\n                  <\/jats:p>","DOI":"10.1096\/fj.202403129rr","type":"journal-article","created":{"date-parts":[[2025,4,4]],"date-time":"2025-04-04T07:41:06Z","timestamp":1743752466000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<scp>STEAP4<\/scp>\n                    facilitates growth, migration, and invasion of prostate carcinoma through upregulation of\n                    <scp>NOTCH4<\/scp>"],"prefix":"10.1096","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3236-3765","authenticated-orcid":false,"given":"Shicheng","family":"Fan","sequence":"first","affiliation":[{"name":"Department of Urology The Third People's Hospital of Yunnan Province  Kunming Yunnan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3205-4903","authenticated-orcid":false,"given":"Zhongyou","family":"Xia","sequence":"additional","affiliation":[{"name":"Department of Urology Beijing Anzhen Nanchong Hospital, Capital Medical University &amp; Nanchong Central Hospital  Nanchong Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-1337-8553","authenticated-orcid":false,"given":"Weijia","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Ultrasound Kunming Maternity and Child Care Hospital  Kunming Yunnan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-5344-7986","authenticated-orcid":false,"given":"Yuanquan","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Urology The Third People's Hospital of Yunnan Province  Kunming Yunnan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6115-3049","authenticated-orcid":false,"given":"Xiaodong","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Urology The First Affiliated Hospital of Kunming Medical University  Kunming Yunnan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9646-172X","authenticated-orcid":false,"given":"Peng","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Urology The First Affiliated Hospital of Kunming Medical University  Kunming Yunnan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-6196-4377","authenticated-orcid":false,"given":"Qingpeng","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Urology The Third People's Hospital of Yunnan Province  Kunming Yunnan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,4,2]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21834"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21820"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.euo.2022.04.007"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27175730"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2022.215927"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2024.03.027"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-021-02146-7"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1530\/JOE-16-0594"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12020-014-0230-1"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40170-023-00323-1"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2019.08.074"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-14698-y"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1712946114"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2021.608201"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm_00000270"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0220456"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2023.110933"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/8060389"},{"key":"e_1_2_11_20_1","unstructured":"National Center for Biotechnology Information.Transcriptomic profiling of Prostate Cancer[EB\/OL].https:\/\/www.ncbi.nlm.nih.gov\/geo\/query\/acc.cgi?acc=GSE229904"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13073-024-01323-6"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz430"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1198\/tech.2002.s656"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.6121"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-12-77"},{"key":"e_1_2_11_26_1","unstructured":"The Comprehensive R Archive Network.survminer: drawing survival curves using \u2018ggplot2\u2019[EB\/OL].https:\/\/rpkgs.datanovia.com\/survminer\/index.html"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1467-985X.2010.00676_9.x"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8"},{"key":"e_1_2_11_29_1","unstructured":"Github.ggradar[EB\/OL].https:\/\/github.com\/ricardo\u2010bion\/ggradar"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/iyad031"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.1.27"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1208677"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2021.108311"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.15252\/emmm.201404181"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.26619"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.38875"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/cas.12978"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10495-017-1368-0"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2016.09.021"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2014.11.013"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1593\/neo.04157"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.1668"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2022.03.022"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-023-01732-y"}],"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.202403129RR","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T17:46:40Z","timestamp":1761673600000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fj.202403129RR"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,2]]},"references-count":43,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2025,4,15]]}},"alternative-id":["10.1096\/fj.202403129RR"],"URL":"https:\/\/doi.org\/10.1096\/fj.202403129rr","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"value":"0892-6638","type":"print"},{"value":"1530-6860","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,2]]},"assertion":[{"value":"2024-12-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70508"}}