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Limited molecular information is available on the intercellular networks that sustain RPE\/choroid tissue in both healthy and pathological states. Galectin\u20101 (Gal1), a \u03b2\u2010galactoside\u2010binding lectin, has recently emerged as a key regulator of angiogenesis and a potential therapeutic target in vascular pathologies, including age\u2010related macular degeneration. Here, we studied the expression of Gal1 in the outer retina and its regulatory role in the RPE\/choroid under physiological and pathological conditions. Our findings indicate that Gal1 is predominantly associated with stromal cells in the RPE\/choroid. In Gal1\u2010deficient (\n                    <jats:italic>Lgals1<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>\u2212\/\u2212<\/jats:italic>\n                    <\/jats:sup>\n                    ) mice, the RPE\/choroid ultrastructure and gene expression profiles were altered, and choroidal explants exhibited reduced sprouting compared to those of wild\u2010type mice. Consistently, recombinant Gal1 promoted choroidal sprouting under hypoxic conditions, and stromal\u2010like cells modulated pro\u2010angiogenic and antiangiogenic gene expression in\u00a0vitro under pathological conditions. Interestingly, Gal1 was also expressed by the RPE, with apical secretion under normoxia that shifted toward a basolateral phenotype under hypoxia. These findings identify stromal\u2010like cells and RPE as key sources of Gal1 in the choroid, highlighting its distinct roles in maintaining RPE\/choroid homeostasis in healthy or pathological microenvironments.\n                  <\/jats:p>","DOI":"10.1096\/fj.202403181r","type":"journal-article","created":{"date-parts":[[2025,5,23]],"date-time":"2025-05-23T11:00:02Z","timestamp":1747998002000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Stromal\u2010Like Cells and Retinal Pigment Epithelium Modulate Choroidal Sprouting Through Galectin\u20101\u2010Dependent and Independent Pathways"],"prefix":"10.1096","volume":"39","author":[{"given":"Magali E.","family":"Ridano","sequence":"first","affiliation":[{"name":"Departamento de Bioqu\u00edmica Cl\u00ednica, Facultad de Ciencias Qu\u00edmicas Universidad Nacional de C\u00f3rdoba  C\u00f3rdoba Argentina"},{"name":"Centro de Investigaciones en Bioqu\u00edmica Cl\u00ednica e Inmunolog\u00eda (CIBICI) Consejo Nacional de Investigaciones Cient\u00edficas y Tecnol\u00f3gicas (CONICET)  C\u00f3rdoba Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christin","family":"Hanke\u2010Gogokhia","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Margaret Dyson Vision Research Institute, Weill Cornell Medicine  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillermo L.","family":"Lehmann","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Margaret Dyson Vision Research Institute, Weill Cornell Medicine  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulo S.","family":"Caceres","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Margaret Dyson Vision Research Institute, Weill Cornell Medicine  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria V.","family":"Vaglienti","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica Cl\u00ednica, Facultad de Ciencias Qu\u00edmicas Universidad Nacional de C\u00f3rdoba  C\u00f3rdoba Argentina"},{"name":"Centro de Investigaciones en Bioqu\u00edmica Cl\u00ednica e Inmunolog\u00eda (CIBICI) Consejo Nacional de Investigaciones Cient\u00edficas y Tecnol\u00f3gicas (CONICET)  C\u00f3rdoba Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danilo","family":"Ceschin","sequence":"additional","affiliation":[{"name":"Instituto Universitario de Ciencias Biom\u00e9dicas de C\u00f3rdoba (IUCBC)  C\u00f3rdoba Argentina"},{"name":"Centro de Investigaci\u00f3n en Medicina Traslacional \u201cSevero R. Amuch\u00e1stegui\u201d (CIMETSA) Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)  C\u00f3rdoba Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7457-6606","authenticated-orcid":false,"given":"Ryan","family":"Schreiner","sequence":"additional","affiliation":[{"name":"Division of Regenerative Medicine, Hartman Institute for Therapeutic Organ Regeneration, Ansary Stem Cell Institute, Department of Medicine Weill Cornell Medicine  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8623-0645","authenticated-orcid":false,"given":"Diego O.","family":"Croci","sequence":"additional","affiliation":[{"name":"Laboratorio de Inmunopatolog\u00eda, Instituto de Histolog\u00eda y Embriolog\u00eda \u201cDr. Mar\u00edo H. 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