{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T08:27:18Z","timestamp":1791620838069,"version":"4.3.4"},"reference-count":49,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,4,29]],"date-time":"2025-04-29T00:00:00Z","timestamp":1745884800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2025,5,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Thyroid carcinoma, with limited efficacy of current treatment, influences the lives and health of many people. It is important to explore potential therapeutic targets for thyroid carcinoma. Fibromodulin (FMOD) has been indicated to be connected with the progression of different kinds of tumors, with unknown functions in thyroid carcinoma. In this study, the potential candidate therapeutic targets for thyroid carcinoma were identified by bioinformatics methods, and FMOD was screened out for verification. Cell counting kit\u20108, wound healing, transwell, and flow cytometry assays were conducted to determine the role of FMOD overexpression in cell viability, migration, invasion, and apoptotic rate of thyroid carcinoma cells, respectively. Subcutaneous tumor growth was monitored in nude mice. Tumor\u2010associated macrophages (TAMs) were co\u2010cultured with thyroid carcinoma cells, and the surface marker of M1\u2010like TAMs, CD80, was identified by flow cytometry. Ras\u2010association proximate 1B (Rap1B), the downstream target of FMOD, was predicted by bioinformatic techniques and validated by Rap1B overexpression rescue. FMOD was identified as a tumor suppressor gene in thyroid carcinoma through bioinformatic techniques. FMOD overexpression inhibited cell viability, migration, and invasion and stimulated apoptosis of thyroid carcinoma cells. In\u00a0vivo, FMOD upregulation could suppress the growth of solid tumors. Moreover, FMOD overexpression in thyroid carcinoma cells promoted M1\u2010like TAM polarization. FMOD downregulated Rap1B expression in thyroid carcinoma cells, and Rap1B overexpression rescue reversed the impact of FMOD on tumor progression and TAM polarization. In conclusion, FMOD exhibited an inhibitory effect on thyroid carcinoma by stimulating M1\u2010like TAM polarization via targeting Rap1B.<\/jats:p>","DOI":"10.1096\/fj.202403290rr","type":"journal-article","created":{"date-parts":[[2025,4,29]],"date-time":"2025-04-29T05:19:44Z","timestamp":1745903984000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Overexpression of\n                    <scp>FMOD<\/scp>\n                    in Thyroid Carcinoma Triggers\n                    <scp>M1<\/scp>\n                    \u2010Like Tumor\u2010Associated Macrophage Polarization by Targeting\n                    <scp>Rap1B<\/scp>"],"prefix":"10.1096","volume":"39","author":[{"given":"Xiangnan","family":"Fang","sequence":"first","affiliation":[{"name":"Department of Endocrinology and Metabolism The First Affiliated Hospital of Gannan Medical University  Ganzhou Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuemei","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolism Gannan Medical University  Ganzhou Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenfeng","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolism Gannan Medical University  Ganzhou Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiming","family":"Lv","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Metabolism The First Affiliated Hospital of Gannan Medical University  Ganzhou Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Ying","sequence":"additional","affiliation":[{"name":"Department of Thyroid Hernia Surgery The First Affiliated Hospital of Gannan Medical University  Ganzhou Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-3265-7351","authenticated-orcid":false,"given":"Jinchang","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Pathology Ganzhou People's Hospital  Ganzhou Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,4,29]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2023.1306232"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12022-021-09666-1"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2021.738213"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(16)30172-6"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345221138525"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12017-024-08793-2"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.26921"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12935-019-0870-6"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00432\u2010023\u201005163\u20106"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2023.108201"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.21890"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.844142"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.583084"},{"issue":"5","key":"e_1_2_11_15_1","first-page":"6217","article-title":"High Density of CD68+ Tumor\u2010Associated Macrophages Predicts a Poor Prognosis in Gastric Cancer Mediated by IL\u20106 Expression","volume":"15","author":"Su C. 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